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GeneArt® Genomic Cleavage Detection Kit

Catalog number: A24372

Invitrogen™  Related applications: Seamless Cloning & Genetic Assembly

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Description

The GeneArt® Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation. When using genome editing tools to obtain targeted mutations, it is necessary to determine the efficiency at which these nucleases cleave the target sequence, particularly prior to proceeding to the more laborious and expensive processes of cloning and sequencing. The GeneArt® genomic cleavage detection (GCD) assay is a relatively quick, simple, and reliable assay that provides the efficiency with which a nuclease cleaves at a given locus.

Easy—no genomic DNA isolation, direct PCR amplification
Rapid—4 hour total processing time
Quantative—gel band density directly correlated to target indel introduction

The GeneArt® genomic cleavage detection kit contains all of the reagents required prior to gel analysis. A sample of the edited cell population is used as a direct PCR template for amplification with primers specific to the targeted region. The PCR product is then denatured and re-annealed to produce heteroduplex mismatches where double strand breaks have occurred, resulting in indel introduction. These mismatches are recognized and cleaved by the detection enzyme. This cleavage is both easily detectable and quantifiable using gel analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 20 reactions
Cloning Method: Restriction Enzyme ⁄ MCS

Contents & storage

Contains:

• 1 bottle Cell Lysis Buffer
• 1 tube Proteinase K
• 1 tube PCR Supermix
• 1 tube Water
• 1 tube Detection Enzyme
• 1 tube Detection Reaction Buffer
• 1 tube Control Template & Primers

Store all at -5 to -30°C.

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