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Invitrogen™

GeneArt™ Genomic Cleavage Detection Kit

Catalog number:  A24372

 Related applications:

Seamless Cloning & Genetic Assembly

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Description

The GeneArt Genomic Cleavage Detection Kit is a fast, T7 endonuclease I based method to quantify how well your genome editing protocol causes insertions and deletions (indels) in the genome of your cell line. This is the quickest way to quantify and validate the best CRISPR-Cas9 gRNA, or the best TAL effector nuclease, for your cell engineering experiment. The GeneArt Genomic Cleavage Detection Kit provides a convenient, quick, and complete solution.

Using this T7 endonuclease I (T7EI) based method, you can quickly and confidently measure your on-target genome editing efficiency generated by non-homologous end joining (NHEJ) activity. The advantages of the GeneArt Genomic Cleavage Detection Kit are:

Minimal hands-on time — PCR amplify from cell lysate, no DNA purification required
Short protocol — four hours from cell harvest to quantified results
Just add PCR primers — everything needed in one box. No separate PCR master mixes or DNA extraction kits to buy.
Quantify editing efficiency directly from your gel — gel band density directly correlates to on-target indel formation

Obtain results the same day without waiting for sequencing results or performing complicated sequencing analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: CRISPR-Cas9, TAL Effector Nuclease
PCR Enzyme: Taq Polymerase
Product Size: 20 reactions
Recognition Site: INDEL

Contents & storage

Contains:

• 1 bottle Cell Lysis Buffer
• 1 tube Proteinase K
• 1 tube PCR Supermix
• 1 tube Water
• 1 tube T7E1 Detection Enzyme
• 1 tube T7E1 Detection Reaction Buffer
• 1 tube Control Template & Primers

Store all at -5 to -30°C.

Documents

Manuals & protocols