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Invitrogen™

GeneArt™ Genomic Cleavage Detection Kit

Catalog number:  A24372

 Related applications: Seamless Cloning & Genetic Assembly

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Description

An essential tool for monitoring the efficiency of your genome editing
The GeneArt Genomic Cleavage Detection Kit is a fast, PCR-based method to measure how well your genome editing tool forms insertion and deletion (indel) mutations. Whether you are trying to identify the best CRISPR gRNA, the best TALEN, or estimate your overall genome editing efficiency, the GeneArt Genomic Cleavage Detection Kit is a convenient, quick, cost-effective solution.

Advantages of the GeneArt Genomic Cleavage Detection Kit:
Minimize your prep time—skip the genomic DNA isolation step typically required by similar kits and directly PCR amplify from cells
Reduce your processing time —four hours from harvest to results
Just add PCR primers—everything needed in one box. No separate PCR master mixes or DNA extraction kits to buy.
Quantitate editing efficiency directly from your gel—gel band density directly correlates to target indel formation

No need to prep samples for sequencing, wait for sequencing results, or upload data to analysis software. With the GeneArt Genomic Cleavage Detection Kit, directly PCR amplify your pool of edited cells with primers specific to your targeted region, run the cleavage assay, and determine efficiency, all in an afternoon.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning Method: Restriction Enzyme ⁄ MCS
Product Line: GeneArt®
Product Size: 20 reactions

Contents & storage

Contains:

• 1 bottle Cell Lysis Buffer
• 1 tube Proteinase K
• 1 tube PCR Supermix
• 1 tube Water
• 1 tube Detection Enzyme
• 1 tube Detection Reaction Buffer
• 1 tube Control Template & Primers

Store all at -5 to -30°C.

Documents

Manuals & protocols