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View additional product information for SimpliAmp™ Thermal Cycler - FAQs (A24811, A35422)
15 product FAQs found
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.
Like the ProFlex PCR System, the SimpliAmp Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.
To add an infinite hold, select the infinity symbol for program.
The SimpliAmp Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:
- PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The maximum block ramp rate of the SimpliAmp Thermal Cycler is 4 degrees C/sec.
The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html).
We offer only a 96-well standard (0.2 mL) block that is not interchangeable.
The two different types of trays are for use with different tubes:
-MicroAmp 96-well Tray/Retainer Set for Veriti Systems (Cat. No. 4381850) - this tray is for use with 0.2 mL single tubes without caps (Cat. Nos. N8010533, N8011533), 0.2 mL 8-tube strip with attached dome caps (Cat. No. A30589), and 0.2 mL tube strips without caps (Cat. No. 4316567)
-MicroAmp 96-well Tray for VeriFlex Blocks (Cat. No. 4379983) - this tray is for use with 0.2 mL single tubes with caps (Cat. Nos. N8010540, N8011540)
The storage capacity for the SimpliAmp Thermal Cycler is approximately 5.9 GB. The run methods and run records stored use most of the memory. It can store 1.2 million run methods.
The SimpliAmp PCR System can be used with a 4 degrees C hold. Further, our in-house QC testing requires the passing of a “no leak” test make sure that any accumulated water (due to condensation when the block temperature is set to low temperature for a long period of time) will not leak into the instrument causing electronic failure.
Unfortunately, we do not sell the sample block for the SimpliAmp Thermal Cycler separately. Only the ProFlex series and the Automated series of thermal cyclers have interchangeable blocks that we sell separately.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.
The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)
The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler