With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can: • Confirm expression of key pluripotent stem cell markers with highly specific antibodies • Get more information per sample by measuring more than one marker at a time • Generate beautiful multiplexed images with minimal effort • Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol • Process samples with confidence using a complete and optimized set of ICC reagents
Confirm Expression of Key PSC Markers The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.
Get More Information Per Sample The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.
Generate Beautiful Multiplexed Images Powered by the superior performance of bright, photostable Alexa Fluor® dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.
Minimize Unnecessary Wash Steps The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.
Process Samples with Confidence An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.
What You Get Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)
For Research Use Only. Not for use in diagnostic procedures.
Store at -20°C to 4°C: • 10 µL of Rabbit Anti-Human OCT4 (200X) • 20 µL of Rat Anti-Human SOX2 (100X) • 20 µL of Mouse Anti-Human SSEA4 (100X) • 20 µL of Mouse Anti-Human TRA-1-60 (100X) • 20 µL of Alexa Fluor® 555 Donkey Anti-Rabbit (250X) • 20 µL of Alexa Fluor® 594 Donkey Anti-Rabbit (250X) • 20 µL of Alexa Fluor® 488 Donkey Anti-Rat (250X) • 20 µL of Alexa Fluor® 555 Goat Anti-Mouse IgM (250X) • 20 µL of Alexa Fluor® 594 Goat Anti-Mouse IgM (250X) • 20 µL of Alexa Fluor® 488 Goat Anti-Mouse IgG3 (250X)
Store at -20°C to RT: • 1 vial of NucBlue® Fixed Cell ReadyProbes® Reagent (1X) • 10 mL of Fixative Solution (1X) • 10 mL of Permeabilization Solution S (1X) • 20 mL of Blocking Solution (1X) • 20 mL of Wash Buffer (10X)