Gibco™

SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

Catalog number:  A2494301

Related applications:

Mammalian Cell Culture

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Description

SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells, which reduces the FBS requirements by 50-90% without any changes in growth rate or morphology. When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using the media selector tool.
This Advanced DMEM/F-12 is manufactured as follows:
WithWithout
• Non-essential amino acids• Glucose
• Sodium pyruvate• Phenol red
 • L-arginine
 • L-glutamine
 • L-lysine
 • HEPES
  

Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System
SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Glucose: No Glucose
Glutamine: No Glutamine
HEPES Buffer: No HEPES
Phenol Red Indicator: No Phenol Red
Product Line: Gibco®
Serum Supplementation: Advanced (Reduced Serum)
Sodium Pyruvate Additive: Sodium Pyruvate
Volume (Metric): 500.0 mL

Contents & storage

Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.