EfficientFeed™ C+ AGT™ Supplement, 10 L - FAQs

What feeding supplementation is recommended for Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504)?

We recommend using EfficientFeed A+, B+, and/or C+ AGT Supplements with Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) to maximize titers. We recommend trying EfficientFeed C+ AGT Supplement (Cat. Nos. A2503101, A2503104, A2503105) and EffiientFeed C+ 2X Supplement (Cat. Nos. A3937601, A3937602) first, and if specific productivity drops off late in culture, try the addition of FunctionMAX TiterEnhancer (Cat. Nos. A1501001, A1501002). Supplements should not be added before half-peak cell density is reached for cultures not supplemented with complete feeding supplements like the EfficientFeed supplements.

Note: When culturing cells without complete feeds for fed-batch production we recommend that you not to allow glucose levels to fall below 2g/L in culture by supplementing with glucose as needed (see the product User Guide for more details).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.