Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4)

Catalog number: A25526

Molecular Probes™  Related applications: Cellular Imaging | Stem Cell Analysis

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Description

The Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) enables optimal image-based analysis of two key markers of human pluripotent stem cells (PSCs): OCT4 and SSEA4. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize PSCs with other marker combinations, we recommend the use of the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).

With the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4), you can:
• Confirm expression of key pluripotent stem cell markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 is a critical nuclear marker, while SSEA4 is an important cell surface marker.

Get More Information Per Sample
The antibodies in the kit have been designed to allow for the specific and simultaneous assessment of OCT4 and SSEA4 along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor® dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.

Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ 488, Alexa Fluor™ 594, Alexa Fluor™ 555, 2 Color
Product Size: 40 tests

Contents & storage

Store at -20°C to 4°C:
• 20 µL of Rabbit Anti-Human OCT4 (100X)
• 20 µL of Mouse Anti-Human SSEA4 (100X)
• 20 µL of Alexa Fluor® 555 Donkey Anti-Rabbit (250X)
• 20 µL of Alexa Fluor® 594 Donkey Anti-Rabbit (250X)
• 20 µL of Alexa Fluor® 488 Goat Anti-Mouse IgG3 (250X)

Store at -20°C to RT:
• 1 vial of NucBlue® Fixed Cell ReadyProbes® Reagent (1X)
• 10 mL of Fixative Solution (1X)
• 10 mL of Permeabilization Solution S (1X)
• 20 mL of Blocking Solution (1X)
• 20 mL of Wash Buffer (10X)

Documents

Manuals & protocols

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