CD44 Alexa Fluor® 488 Conjugate Kit for Live Cell Imaging

Catalog number: A25528

Molecular Probes™  Related applications: Cellular Imaging | Stem Cell Analysis

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Description

Molecular Probes® human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This CD44 Alexa Fluor® 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the negative PSC marker CD44. For fixed cell staining of PSC markers and germ layer markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit and the 3-Germ Layer Immunocytochemistry Kit.

With the CD44 Alexa Fluor® 488 Conjugate Kit for Live Cell Imaging, you can:
• Detect the expression of a negative pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM imaging medium
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants
• Combine with additional markers to get more information per sample

Detect Expression of a Negative PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of CD44. CD44 is absent in PSCs and expressed on the surface of many other cell types, including human and mouse fibroblasts. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM.

Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.

Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.

Combine with Other Markers
To get more information per sample, combine this kit with the TRA-1-60 Alexa Fluor® 555 Conjugate Kit for Live Cell Imaging or the TRA-1-60 Alexa Fluor® 594 Conjugate Kit for Live Cell Imaging.

What You Get
Easily scaled to any culture plate, dish, or slide format, the CD44 Alexa Fluor® 488 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 µL staining volume and includes:
• 200 µL of Alexa Fluor® 488 Rat Anti-Human and Mouse CD44 (50X concentration)
• 500 mL FluoroBrite™ DMEM
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ 488
Product Size: 50 tests

Contents & storage

• 200 µL of Alexa Fluor® 488 Rat Anti-Human and Mouse CD44 (50x concentration) (store at 2–8°C)
• 500 mL FluoroBrite™ DMEM (store at 2–8°C, protect from light)

Documents

Manuals & protocols

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