Invitrogen™

Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Green features
Catalog number: A25601
Invitrogen™

Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Green features
Catalog number: A25601
Catalog Number
A25601
Unit Size
20 preps
Price (USD)
295.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
400 preps
Price (USD)
4,630.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 preps
Price (USD)
1,438.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A2560120 preps
295.00
Your Price:
***
A25599400 preps
4,630.00
Your Price:
***
A25600100 preps
1,438.00
Your Price:
***
Product Overview
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

This new addition to the family includes a Power SYBR™ Green 1-Step RT-PCR master mix. The 1-step format of the RT-PCR step streamlines the Cells-to- CT™ 'sample to answer' workflow more than ever without compromising sensitivity and specificity and at an extraordinary value.

The Cells-to-CT 1-Step Power SYBR™ Green Kit is:
• Complete—optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR™ Green 1-Step RT-PCR Master Mix
• Faster—7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
• Simple—12 steps total (sample prep and real-time RT-PCR combined)
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Affordable—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample prepared; study multiple targets per sample at an affordable price

Simple 7-minute Sample Preparation, Then Ready for Real-time RT-PCR
Whether you are using plates or tubes, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit uses a simple 7-minute sample preparation procedure. The lysis technology is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature with a simultaneous DNase treatment. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysate is now ready to analyze on your real-time PCR instrument. Simply add it to a real-time RT-PCR master mix containing the primer sets for your genes of interest. Alternatively, the lysate can be stored at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

Completely Validated, Completely Affordable
All components of the Cells-to-CT™ 1-Step Power SYBR™ Green Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation and real-time 1-step RT-PCR master mixes.

For added quality assurance, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit has been validated with a set of primers for common gene targets and shows performance equivalent to that obtained with purified RNA.

When you add it all up, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit is the best value for your gene expression experiments. With enough reagents to perform 5 real-time RT-PCRs per sample, the savings are substantial compared to buying master mix and an RNA purification kit separately to do the same work.

Accessory Product
The SYBR™ Green Cells-to-CT™ Control Kit was designed for use with SYBR™ Green Cells-to-CT™ products. This kit contains an endogenous control primer set (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding primer set to monitor the effects of PCR inhibition.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

Specifications

Detection Method
SYBR
Format
Tube
GC-Rich PCR Performance
High
PCR Method
1-Step qRT-PCR
Reaction Speed
Standard
Sample Type
Cells, RNA
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Green Features
Fewer resources used and less waste, Less hazardous
Includes
Lysis Solution, 2.2mL; DNase I, 22μL; Stop Solution, 200μL; Power SYBR Green 1-Step qPCR Mix, 500μL; Power SYBR Green 1-Step RT Mix, 10μL
Product Line
Cells-to-CT™, Power SYBR™ Green
Product Type
1-Step qPCR kit

Contents & Storage

• Lysis Solution, 2.2 mL, store at 2–8°C
• Dnase I, 22 µL, store at -5 to -30°C
• Stop Solution, 200 µL, store at -5 to -30°C
• Power SYBR™ Green 1-Step qPCR Mix, 500 µL, store at -5 to -30°C
• Power SYBR™ Green 1-Step RT Mix, 10 µL, store at -5 to -30°C

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