Invitrogen™

Cells-to-CT™ 1-Step TaqMan™ Kit

Green features
Catalog number: A25602
Invitrogen™

Cells-to-CT™ 1-Step TaqMan™ Kit

Green features
Catalog number: A25602
Catalog Number
A25602
Unit Size
400 preps
Price (USD)
6,330.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 preps
Price (USD)
1,778.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
20 preps
Price (USD)
366.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A25602400 preps
6,330.00
Your Price:
***
A25603100 preps
1,778.00
Your Price:
***
A2560520 preps
366.00
Your Price:
***
Product Overview
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

This new addition to the family includes a TaqMan™ 1-Step RT-PCR master mix. The single-tube format of the RT-PCR step streamlines the “sample to answer” workflow more than ever. Combining the simple Cells-to- CT™ lysis technology with the fast cycling capability of the 1-step RT-PCR master mix allows quicker time to results with limited sample handling.

The Cells-to-CT™ 1-Step TaqMan™ Kit is:
• Complete—optimized workflow includes cell lysis reagents with gDNA removal and TaqMan™ 1-Step RT-PCR Master Mix
• Faster than ever—7-minute sample prep, including DNase treatment, combined with a 28-minute real-time RT-PCR step allows for ∼35 minute sample-to-answer workflow
• Simple—10 steps total (sample prep and real-time RT-PCR combined)
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Convenient—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample you prepare; this allows you to study multiple targets or perform replicate RT-PCRs

Note: TaqMan™ primer/probe sets are sold separately.

Simple 7-minute Sample Preparation, Then Ready for Real-time RT-PCR
Whether you are using plates or tubes, the Cells-to-CT™ 1-Step TaqMan™ Kit uses a simple 7-minute sample preparation procedure. The lysis technology is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature with a simultaneous DNase treatment. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysate is now ready to analyze on your real-time PCR instrument. Simply add it to a real-time RT-PCR master mix containing the TaqMan™ primer/probe sets of interest. Alternatively, the lysate can be stored at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

A Complete, Validated Solution
All components of the Cells-to-CT™ 1-Step TaqMan™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation and real-time 1-step RT-PCR master mixes. For added quality assurance, the Cells-to-CT™ 1-Step TaqMan™ Kit has been validated with TaqMan™ Gene Expression assays and shows performance equivalent to that obtained with purified RNA.

Accessory Product
The TaqMan™ Cells-to-CT™ Control Kit was designed for use with TaqMan™ Cells-to-CT™ products. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TaqMan™ Gene Expression assay to monitor the effects of PCR inhibition.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Primer-Probe Detection
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
400 Reactions
PCR Method
1-step RT-qPCR
Reaction Speed
Fast, Standard
Sample Type
Cells, RNA
Volume
22 mL
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Green Features
Fewer resources used and less waste, Less hazardous
Product Line
Cells-to-CT™, TaqMan™
Product Type
1-Step qPCR kit

Contents & Storage

• Lysis Solution, 22 mL, store at 2–8°C
• Dnase I, 220 µL, store at -5 to -30°C
• Stop Solution, 2 x 1 mL, store at -5 to -30°C
• TaqMan™ 1-Step qRT-PCR Mix, 10 mL, store at -5 to -30°C

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