Precision ID Identity Panel

Catalog number: A25643

Applied Biosystems™  Related applications: DNA Sequencing | Forensics | Ion Torrent™ Next-Generation Sequencing

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The Applied Biosystems™ Precision ID Identity Panel is one of the first next-generation sequencing (NGS) solutions for human identification that uniquely identifies pristine or degraded samples. The multiplexed panel of SNPs provides discrimination of individuals similar to STR genotype match probabilities used by forensic analysts (between 1x10-31 and 6x10-35).

High discrimination power is achieved by using 34 upper Y-Clade SNPs and 90 autosomal SNPs that have high heterozygosity and low fixation index (Fst) as described in publications by Dr. Kenneth Kidd from Yale University and the SNPForID Consortium (1-3). The panel design has been optimized for use with degraded or challenging forensic samples using only 1 ng of starting DNA input, although acceptable performance has been observed with sub-nanogram input quantities as low as 180 pg. The autosomal markers have an average amplicon length of 132 nucleotides and the Y-markers are 141 nucleotides in length on average. Using a small sample input of as little as 1 ng of DNA, you can go from sample extraction to genotypes in less than a two days using the Precision ID NGS System and HID SNP Genotyper plug-in for analysis.

• Small amplicons enable better recovery with degraded samples
• As little as 1 ng of input
• Automated library and template preparation using the Ion Chef™ System

• Multiple chip formats to meet a range of sample throughput requirements
• Ability to multiplex up to 264 samples*

• Less than 45 minutes of hands-on time for a DNA-to-data targeted sequencing workflow*
• Total sample-to-data time of less than 2 days*

* Using the Applied Biosystems Precision ID NGS System for Human Identification

1. Pakstis, AJ, Speed, WC, Fang, R, Hyland, FC, Furtado, MR, Kidd, JR, and Kidd, KK. (2010) SNPs for a universal individual identification panel. Human Genetics 127(3), 315-324.
2. Phillips, C, Fang, R, Ballard, D, Fondevila, M, Harrison, C, Hyland, F, et al. (2007) Evaluation of the Genplex SNP typing system and a 49plex forensic marker panel. Forensic Science International: Genetics 1(2), 180-185.
3. Karafet, TM, Mendez, FL, Meilerman, MB, Underhill, PA, Zegura, SL, and Hammer, MF. (2008) New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree. Genome Research 18(5), 830-838.
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System, Ion OneTouch™ 2 System, Ion Chef™ System, Ion S5™ System, Ion S5™ XL System
Library Type: Targeted Sequencing Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 96 reactions
Final Product: PCR Amplicons

Contents & storage

Store in freezer (-5 to -30°C).


Manuals & protocols