For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.
Features of the Expi293™ MembranePro™ Expression System include: • Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system • Scalable—easy scale-up without the need for multiple flasks • Easy to use—no enzymatic or mechanical dissociation required
In addition, benefits of MembranePro™ technology include: • Proteins are displayed on human (or other mammalian) cell membranes • Cellular quality control and mammalian post-translational processing helps produce functional protein • Proteins bud off as lipoparticles and are easily collected from the culture medium • Lipoparticles are enriched with expressed receptors
With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will: 1. Clone your gene of interest using a TOPO® vector 2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent 3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix 4. Store lipoparticles at -80 C or proceed to downstream assays
Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO® Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.
For Research Use Only. Not for use in diagnostic procedures.
Virus-Like Particles (VLPs)
Membrane Protein Expression
Contents & storage
• MembranePro™ Reagent, 1 x 0.3 mg (1 mg/mL), store at -5 to -30°C • MembranePro™ Precipitation Mix, 1 x 75 mL, store at room temperature • 1 ExpiFectamine™ 293 transfection kit (containing reagent and enhancers) for 1 L of culture, store at 2–8°C • Opti-MEM® I Reduced Serum Medium, 1 x 100 mL, store at 2–8°C • Expi293™ Expression Medium, 1 x 1 L, store in the dark at 2–8°C