Expi293™ MembranePro™ Expression System

Name: Expi293™ MembranePro™ Expression System
SKU: A25870

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membraneRead more

Change view

Catalog Number	Quantity
A25870	100 Reactions
A25869	10 Reactions

2 Options

Catalog number A25870

Price (USD)

Quantity:

100 Reactions

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.

Features of the Expi293™ MembranePro™ Expression System include:
• Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system
• Scalable—easy scale-up without the need for multiple flasks
• Easy to use—no enzymatic or mechanical dissociation required

In addition, benefits of MembranePro™ technology include:
• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian post-translational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO™ vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell LineExpi293F™

Delivery TypeVirus-Like Particles (VLPs)

For Use With (Application)Membrane Protein Expression

Product LineExpi293, MembranePro

Quantity100 Reactions

Sufficient For100 Reactions

PromoterEF-1α

TypeExpression System

Unit SizeEach

Contents & Storage

• MembranePro™ Reagent, 10 x 0.3 mg (1 mg/mL), store at -5 to -30°C
• MembranePro™ Precipitation Mix, 10 x 75 mL, store at room temperature
• 10 ExpiFectamine™ 293 transfection kits (containing reagent and enhancers) each for 1 L of culture, store at 2–8°C
• Opti-MEM™ I Reduced Serum Medium, 10 x 100 mL, store at 2–8°C
• Expi293™ Expression Medium, 10 x 1 L, store in the dark at 2–8°C

Frequently asked questions (FAQs)

The Expi293F cells supplied with the Expi293 MembranePro Expression System had poor viability upon thawing. Can I use a different cell line?

While other cell lines can be tested, we recommend using the Expi293F cells, as the performance of the kit has been optimized for use with this cell line (i.e., transfection efficiency using ExpiFectamine Transfection Reagent).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can I use a different expression vector with the MembranePro Functional Protein Expression Kit or the Expi293 MembranePro Expression System?

While other expression vectors and promoters can be tested, we recommend using the pEF6 V5-His TOPO TA expression vector for optimal yields, as the performance of these kits has been optimized for use with this expression vector containing the EF-1alpha promoter.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is there a positive control included with the MembranePro Functional Protein Expression Kit or the Expi293 MembranePro Expression System?

No. However, you can visually tell if MembranePro particles formed via a pellet at the bottom of the tube following precipitation. You can also test the function of your MembranePro particles via receptor-ligand binding studies.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am interested in expressing some functional membrane proteins in mammalian cells. Do you offer a system that will allow me to do this?

We offer the MembranePro Functional Protein Expression System for the expression and display of mammalian cell surface membrane proteins, including G-protein coupled receptors (GPCRs), in an aqueous-soluble format. The system uses virus-like particles (VLPs) to capture lipid raft regions of the cell’s plasma membrane as the VLPs are secreted from the cell. Using this system, it is possible to capture and display endogenous or overexpressed GPCRs and other cell surface membrane proteins in their native context for downstream assays. Since the VLPs are packaged by the cell and secreted into the culture medium, VLPs allow for the isolation of functional membrane proteins by simply decanting and clarifying the culture medium, and isolating the VLPs by precipitation. This represents a substantial savings in time, effort, and required machinery over preparing cell membrane fractions. Because VLPs capture receptor-rich regions of the plasma membrane, your GPCR may also be substantially enriched over crude membrane preparations.

The standard MembranePro Functional Protein Expression System is offered in two configurations: The MembranePro Functional Protein Expression Kit (Cat. No. A11667), which provides the convenience of a complete kit with all the reagents supplied for 10 reactions, and the MembranePro Functional Protein Support Kit, which includes the reagents for functional expression of membrane proteins but does not contain the expression vector cloning module or the 293FT host cells. The MembranePro Functional Protein Support Kit is offered in three sizes, allowing 10, 60, or 600 reactions (Cat. Nos. A11668, A11669, A11670, respectively).

For high-yield expression of functional membrane proteins in Exp293F cells, we offer the Expi293 MembranePro Expression System (Cat. Nos. A25869, A25870) that combines the scalability and ease of use of Expi293 and the technology of MembranePro to allow an increase of more than 20-fold in membrane protein yield compared to the standard, adherent culture MembranePro Functional Protein Expression System. Please see the Application Note for more details.

Note: Expi293F cells (Cat. No. A14527) and pEF6 V5 His TOPO Expression Vector Kit (Cat. No. K961020) are not supplied with the Expi293 MembranePro Expression System and have to be purchased separately as required.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I have misplaced the pEF6 V5-His TOPO TA expression vector from the Expi293 MembranePro Expression System. Can I use a different expression vector?

While other expression vectors and promoters can be tested, we recommend using the pEF6 V5-His TOPO TA expression vector for optimal yields, as the performance of the kit has been optimized for use with this expression vector containing the EF-1alpha promoter.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all Expi293™ MembranePro™ Expression System, 100 Reactions FAQs