Dynamis™ Medium, 1000 mL - FAQs

Dynamis Medium contains glucose at a concentration of 6 g/L.

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While the full formulation for Dynamis Medium is proprietary information that cannot be disclosed, we can confirm that the medium contains a surfactant and that no additional surfactant would need to be added to the medium to support your suspension CHO cells in shake flask culture.

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CD FortiCHO medium has proven to be an excellent single-stream medium for CHO production clone development and banking. In our experience, CHO clones created and selected in CD FortiCHO medium can be put directly into Dynamis medium without any adaptation. In fact, our work suggests that those cells will produce as well or better in Dynamis medium and are just as stable in Dynamis medium if used for process development and scale-up.

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We have done some studies to show comparability of results between the two media, and that information may be filed with agencies in support of any work your Regulatory Team feels is necessary to establish transitioning from the one medium to the other. It is possible that characterization of your expressed protein after you see no other titer changes will be viewed no differently than a CMC update or minor modification to feeding profile during technical transfer to manufacturing. Of course our Regulatory Team would be happy to have a discussion with your team if it makes this transition any simpler for you.

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When culturing cells without complete feeds for fed-batch production, we recommend that you do not to allow glucose levels to fall below 2 g/L in culture by supplementing with glucose as needed (see product insert for more details). We have found that EfficientFeed A+, B+, and/or C+ supplements work well with Dynamis medium to maximize titers. We recommend trying EfficientFeed C+ 1X and 2X first, and if specific productivity drops off late in culture try the addition of FunctionMAX Titer Enhancer. No supplements should be added before reaching the day when half-peak cell density is reached for cultures not fed with complete feeding supplements like the EfficientFeed supplements.

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When using a CHO cell line other than Lonza's GS Gene Expression System, addition of 2-8 mM L-glutamine or similar amount of GlutaMAX Supplement is recommended. Additionally, we recommend that you do not allow glucose levels to fall below 2 g/L in culture by supplementing with glucose as needed (see product insert for more details).

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Dynamis medium was designed to be more scalable in AGT dry format than CD FortiCHO liquid medium. Although there are many formulation similarities, the key differences are equimolar substitutions of alternate forms of some components as well as addition of a small amount of a component found in many other basal media.

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