Rapid transport of internalized P-selectin to late endosomes and the TGN: roles in regulating cell surface expression and recycling to secretory granules.
AuthorsStraley KS, Green SA
JournalJ Cell Biol
PubMed ID11018057
'Prior studies on receptor recycling through late endosomes and the TGN have suggested that such traffic may be largely limited to specialized proteins that reside in these organelles. We present evidence that efficient recycling along this pathway is functionally important for nonresident proteins. P-selectin, a transmembrane cell adhesion protein involved ... More
Targeting a neuropeptide to discrete regions of the motor arborizations of a single neuron.
AuthorsKueh D, Jellies JA,
JournalJ Exp Biol
PubMed ID22623199
'The heart excitor (HE) motor neuron in the leech Hirudo releases acetylcholine (ACh) and a peptide, FMRFamide, to regulate the contractile activity of the heart tube and associated side vessels. Consistent with Dale''s principle, it was assumed that both neurotransmitters were localized to all presynaptic varicosities. However, we found discrete ... More
Intersectin-2L regulates caveola endocytosis secondary to Cdc42-mediated actin polymerization.
AuthorsKlein IK, Predescu DN, Sharma T, Knezevic I, Malik AB, Predescu S,
JournalJ Biol Chem
PubMed ID19622753
'Here we addressed the role of intersectin-2L (ITSN-2L), a guanine nucleotide exchange factor for the Rho GTPase Cdc42, in the mechanism of caveola endocytosis in endothelial cells (ECs). Immunoprecipitation and co-localization studies showed that ITSN-2L associates with members of the Cdc42-WASp-Arp2/3 actin polymerization pathway. Expression of Dbl homology-pleckstrin homology (DH-PH) ... More
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
'The identification of alternative splice variants of Survivin that possess distinct functions from those originally identified for the main Survivin isoform has greatly increased the complexity of our understanding of the role of Survivin in different cells. Previous functional studies of the Survivin splice variants have been performed almost exclusively ... More
Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.
Authorsvan Roy N, Mangelschots K, Speleman F
JournalTrends Genet
PubMed ID8488564
Acylation of CD44 and its association with lipid rafts are required for receptor and hyaluronan endocytosis.
AuthorsThankamony SP, Knudson W,
JournalJ Biol Chem
PubMed ID16945930
CD44 is a cell surface receptor for the extracellular matrix macromolecule hyaluronan. In addition, CD44 mediates the endocytosis of hyaluronan leading to its subsequent degradation within lysosomes. Using model systems of COS-7 and Flp-293 cells, we demonstrate that the association of CD44 with lipid rafts is essential for the endocytosis ... More
Targeting aberrant sialylation in cancer cells using a fluorinated sialic acid analog impairs adhesion, migration, and in vivo tumor growth.
AuthorsBüll C, Boltje TJ, Wassink M, de Graaf AM, van Delft FL, den Brok MH, Adema GJ,
Journal
PubMed ID23974695
Cancer cells decorate their surface with a dense layer of sialylated glycans by upregulating the expression of sialyltransferases and other glycogenes. Although sialic acids play a vital role in many biologic processes, hypersialylation in particular has been shown to contribute to cancer cell progression and metastasis. Accordingly, selective strategies to ... More
L-Dopa activates histaminergic neurons.
AuthorsYanovsky Y, Li S, Klyuch BP, Yao Q, Blandina P, Passani MB, Lin JS, Haas HL, Sergeeva OA,
JournalJ Physiol
PubMed ID21242252
L-Dopa is the most effective treatment of early and advanced stages of Parkinson's disease (PD), but its chronic use leads to loss of efficiency and dyskinesia. This is delayed by lower dosage at early stages, made possible by additional treatment with histamine antagonists. We present here evidence that histaminergic tuberomamillary ... More
Alterations of splenic architecture in malaria are induced independently of Toll-like receptors 2, 4, and 9 or MyD88 and may affect antibody affinity.
AuthorsCadman ET, Abdallah AY, Voisine C, Sponaas AM, Corran P, Lamb T, Brown D, Ndungu F, Langhorne J,
JournalInfect Immun
PubMed ID18559428
Splenic microarchitecture is substantially altered during acute malaria infections, which may affect the development and regulation of immune responses. Here we investigated whether engagement of host Toll-like receptor 2 (TLR2), TLR4, TLR9, and the adaptor protein MyD88 is required for induction of the changes and whether antibody responses are modified ... More
Recurrent collateral connections of striatal medium spiny neurons are disrupted in models of Parkinson's disease.
AuthorsTaverna S, Ilijic E, Surmeier DJ,
JournalJ Neurosci
PubMed ID18495884
The principal neurons of the striatum, GABAergic medium spiny neurons (MSNs), are interconnected by local recurrent axon collateral synapses. Although critical to many striatal models, it is not clear whether these connections are random or whether they preferentially link functionally related groups of MSNs. To address this issue, dual whole ... More
Evaluation of an 111In-radiolabeled peptide as a targeting and imaging agent for ErbB-2 receptor expressing breast carcinomas.
AuthorsKumar SR, Quinn TP, Deutscher SL,
JournalClin Cancer Res
PubMed ID17947470
The cellular targeting and tumor imaging properties of a novel ErbB-2-avid peptide, discovered from bacteriophage display, were evaluated in human breast carcinoma cells and in breast carcinoma-xenografted mice. The affinity of the ErbB-2 targeting peptide KCCYSL and its alanine substituted counterparts for the extracellular domain (ECD) of purified recombinant ErbB-2 ... More
HIV-1 Nef disrupts antigen presentation early in the secretory pathway.
AuthorsKasper MR, Roeth JF, Williams M, Filzen TM, Fleis RI, Collins KL,
JournalJ Biol Chem
PubMed ID15653685
Human immunodeficiency virus, type 1 Nef disrupts viral antigen presentation and promotes viral immune evasion from cytotoxic T lymphocytes. There is evidence that Nef acts early in the secretory pathway to redirect major histocompatibility complex class I (MHC-I) from the trans-Golgi network to the endolysosomal pathway. However, a competing model ... More
Intersectin regulates fission and internalization of caveolae in endothelial cells.
AuthorsPredescu SA, Predescu DN, Timblin BK, Stan RV, Malik AB,
JournalMol Biol Cell
PubMed ID12960435
Intersectin, a multiple Eps15 homology and Src homology 3 (SH3) domain-containing protein, is a component of the endocytic machinery in neurons and nonneuronal cells. However, its role in endocytosis via caveolae in endothelial cells (ECs) is unclear. We demonstrate herein by coimmunoprecipitation, velocity sedimentation on glycerol gradients, and cross-linking that ... More
Peptides specific to the galectin-3 carbohydrate recognition domain inhibit metastasis-associated cancer cell adhesion.
AuthorsZou J, Glinsky VV, Landon LA, Matthews L, Deutscher SL,
JournalCarcinogenesis
PubMed ID15528216
Intravascular cancer cell adhesion plays a significant role in the metastatic process. Studies indicate that galectin-3, a member of the galectin family of soluble animal lectins, is involved in carbohydrate-mediated metastatic cell heterotypic (between carcinoma cells and endothelium) and homotypic (between carcinoma cells) adhesion via interactions with the tumor-specific Thomsen-Friedenreich ... More
Leukocyte-inspired biodegradable particles that selectively and avidly adhere to inflamed endothelium in vitro and in vivo.
AuthorsSakhalkar HS, Dalal MK, Salem AK, Ansari R, Fu J, Kiani MF, Kurjiaka DT, Hanes J, Shakesheff KM, Goetz DJ,
JournalProc Natl Acad Sci U S A
PubMed ID14668435
We exploited leukocyte-endothelial cell adhesion chemistry to generate biodegradable particles that exhibit highly selective accumulation on inflamed endothelium in vitro and in vivo. Leukocyte-endothelial cell adhesive particles exhibit up to 15-fold higher adhesion to inflamed endothelium, relative to noninflamed endothelium, under in vitro flow conditions similar to that present in ... More
Evaluation of prototype transmembrane 4 superfamily protein complexes and their relation to lipid rafts.
AuthorsClaas C, Stipp CS, Hemler ME,
JournalJ Biol Chem
PubMed ID11113129
Recent literature suggests that tetraspanin proteins (transmembrane 4 superfamily; TM4SF proteins) may associate with each other and with many other transmembrane proteins to form large complexes that sometimes may be found in lipid rafts. Here we show that prototype complexes of CD9 or CD81 (TM4SF proteins) with alpha(3)beta(1) (an integrin) ... More