resDNASEQ® Human Residual DNA Quantitation Kit with PrepSEQ® Residual DNA Sample Preparation Kit

Catalog number: A27335

Applied Biosystems™  Related applications: Contaminant & Impurity QC Testing

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The resDNASEQ® Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins. Reliable and rapid, the resDNASEQ® system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of human cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ® Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone without the sample preparation kit, please see resDNASEQ® Human Residual DNA Quantitation Kit.

• Highly sensitive quantitation using proven TaqMan® real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan® primer/probe mix, and human genomic DNA standard

The resDNASEQ® Human Residual DNA Quantitation Kit provides highly sensitive detection of human DNA, allowing you to use small sample volumes to generate accurate results. The broad linear range of TaqMan® real-time qPCR technology allows you to test samples with variable levels of human DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use
With the resDNASEQ® Human Residual DNA Quantitation Kit you perform qPCR analysis to compare the amount of human DNA in your test samples to a standard curve generated with known amounts of purified human genomic DNA standard. The resDNASEQ® kit includes almost everything you need to carry out these steps, from master mix to TaqMan® primer/probe mix to human genomic DNA standard, as well as step-by-step instructions.

Consistent performance
For accurate quantitation of residual human DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ® system you get consistent assay performance across a range of DNA fragment sizes.
Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.


Host Cell: Human cell lines
Product Size: 100 reactions
Detection Method: Fluorescent
Shipping Condition: Dry Ice

Contents & storage

resDNASEQ® Human DNA Real-Time PCR Reagents:
• 2X Environmental Master Mix (2 tubes, 0.75 mL per tube). Store at -5 to -30°C before first use, protect from light.
• 10X Human DNA Assay Mix (1 tube, 300 µL). Store at -5 to -30°C, protect from light.
• Negative control water (1 tube, 1.0 mL). Store at -5 to -30°C before first use and 2–8°C after first use.

resDNASEQ® Human DNA Control:
• Human DNA Control (1 tube, 40 µL, 30 ng/µL). Store at -5 to -30°C.
• DNA Dilution Buffer (1 bottle, 7 mL). Store at -5 to -30°C before first use and 2–8°C after first use.

PrepSEQ® Nucleic Acid Extraction Kit:
• Lysis Buffer (2 bottles, 50 mL/bottle).
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/bottle).
• Elution Buffer (1 bottle, 25 mL).
• Proteinase K Buffer( 1 bottle, 50 mL).
• Magnetic Particles (2 tubes, 1.5 mL/tube. Store at 2–8°C.
• Proteinase K (20 mg/mL) (1 tube, 1.25 mL). Store at or below -20°C.
Store all at room temperature unless otherwise indicated.

PrepSEQ® REsidual DNA Sample Preparation Kit:
• Proteinase K (20 mg/mL) (1 tube, 1.25 mL).
• Yeast tRNA (10 mg/mL) (1 tube, 0.5 mL).
• Glycogen (5 mg/mL) (2 tubes, 1.0 mL/tube).
Store all at or below -20°C.


Manuals & protocols

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