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Applied Biosystems™

ProteinSEQ™ CHO HCP Quantitation Kit

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Description

The ProteinSEQ™ CHO HCP Quantitation Kit is an assay kit containing all reagents and buffers needed to perform 200 PCR reactions to quantify process-independent host cell proteins from CHO cell lines. It combines a broadly reactive anti-CHO HCP antibody with a qPCR-based TaqMan™ Assay platform to enable measurements of HCPs with high sensitivity and wide dynamic range.

The ProteinSEQ CHO HCP Quantitation Kit contains:

• Capture beads loaded with anti-CHO HCP antibody
• CHO HCP standards
• All reagents and buffers needed to perform 200 PCR reactions for the process-independent quantitation of host cell proteins

Features include:

• Precise—quantitate host cell protein contaminants with excellent precision
• Fast—helps accelerate process development with more valid test results per run
• Efficient—helps reduce hands-on time with less sample dilution and preparation
• Reproducible—achieve unprecedented productivity with 4-log dynamic range

ProteinSEQ CHO HCP quantitation
The ProteinSEQ assay platform uses qPCR technology to measure protein process contaminants with extraordinary sensitivity and unparalleled dynamic range. Host cell protein contaminants from CHO cell lines can be accurately measured from 0.5–3,150 ng/mL, with outstanding quantitation efficiency and dilutional linearity.

Streamlined workflow
The ProteinSEQ CHO HCP Quantitation Kit is the only instrument platform-based HCP quantitation solution to deliver a generic, process-independent HCP assay with class-leading sensitivity and 4-log dynamic range. The kit helps accelerate laboratory workflows because the number of sample dilutions and replicates is reduced in each run.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Protein Quantitation Assay
Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast System
Product Line: ProteinSEQ™
Shipping Condition: Approved for shipment on Wet or Dry Ice
Technique: Proximity Ligation Assay

Contents & storage

100 mL ProteinSEQ Wash Buffer, store at 2–8°C
50 mL CHO HCO ProteinSEQ Diluent, Store at 2–8°C
7 mL ProteinSEQ Elution Buffer, store at 2–8°C
5.5 mL CHO HCP Capture Beads, store at 2–8°C
6 mL Fast Master Mix (2X), store at 2–8°C
250 µL CHO HCP Standards, store at -5 to -30°C
350 µL CHO HCP 3' Probe, store at -5 to -30°C
350 µL CHO HCP 5' Probe, store at -5 to -30°C
600 µL ProteinSEQ Ligation and Assay Mix, store at -5 to -30°C
55 µL ProteinSEQ Ligase, store at -5 to -30°C