Please note: On September 15th, 2016, this product will be discontinued and replaced by equivalent product A3062601.

PSC Definitive Endoderm Induction Kit (Prototype)

Catalog number: A27654SA

Gibco™  Related applications: Cell Viability, Proliferation & Function | Stem Cell Culture | Stem Cell Differentiation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Note: The "prototype" status of this product means that the product is currently undergoing real-time stability studies in compliance with cGMP regulations.

Gibco® PSC Definitive Endoderm Induction Kit consists of two xeno-free media that enable efficient induction of human pluripotent stem cells (PSCs) to definitive endoderm. Unlike other methods that require multiple components and take five or more days, the PSC Definitive Endoderm Induction Kit can generate ≥90% CXCR4+/PDGFRα- definitive endoderm cells with only two components in just two days. Each medium is supplied as a 1X complete medium, requiring no mixing of additional components, and the resultant definitive endoderm is capable of differentiating to midgut/hindgut, pancreatic endoderm, or liver bud progenitors that express relevant physiological markers.

Generation of highly pure definitive endoderm
The PSC Definitive Endoderm Induction Kit is capable of producing highly pure populations of definitive endoderm from pluripotent stem cells, showing ≥90% CXCR4+/PDGFRα- across multiple lines and high expression of key markers SOX17 and FOXA2.

Generation of high quality definitive endoderm
Definitive endoderm generated using PSC Definitive Endoderm Induction Kit is capable of differentiating to downstream lineages such as midgut/hindgut (expressing CDX2), pancreatic endoderm (expressing PDX2), and liver bud progenitors (expressing AFP).

Rapid production of definitive endoderm
The PSC Definitive Endoderm Induction Kit is capable of producing definitive endoderm in only two days.

No mixing of components required
Each medium is supplied at a 1X concentration, requiring no mixing of reagents. Simply thaw the media and add to PSC culture.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells (Human)
Product Size: 1 kit
Shipping Condition: Dry Ice

Contents & storage

1 x 50 mL PSC Definitive Endoderm Induction Medium A
1 x 50 mL PSC Definitive Endoderm Induction Medium B

Store at -5 to -30°C.

Documents

Manuals & protocols

Recommended products