Ion 520™ Chip Kit - FAQs

View additional product information for Ion 520™ Chip Kit - FAQs (A27762)

28 product FAQs found

How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?

The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the maximum throughput/day for the Ion S5 GeneStudio Prime System?

The Ion S5 GeneStudio Prime System has a maximum throughput/day of 50 Gb (2 Ion 550 Chips).

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How long does it take to run an Ion 550 Chip on the Ion S5 GeneStudio Prime System?

The Ion S5 GeneStudio Prime System takes 8.5 hours to run an Ion 550 Chip.

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How long does it take to run an Ion 540 Chip on the Ion S5 GeneStudio Prime System?

The Ion S5 GeneStudio Prime System takes 6.5 hours to run an Ion 540 Chip.

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What chips are compatible with the Ion GeneStudio S5 Prime System?

Ion 510 Chip, Ion 520 Chip, Ion 530 Chip, Ion 540 Chip, and Ion 550 Chip are compatible with the Ion GeneStudio S5 Prime System.

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Is the Ion GeneStudio S5 Prime System compatible with the Ion One Touch 2 System?

Yes, the Ion GeneStudio S5 Prime System compatible with the Ion One Touch 2 System.

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Is the Ion GeneStudio S5 Prime System compatible with the Ion Chef System?

Yes, the Ion GeneStudio S5 Prime System is compatible with the Ion Chef System.

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Does the Ion GeneStudio S5 Prime System have a separate server?

Yes, the Ion GeneStudio S5 Prime System has a separate server.

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What is the server storage size on the Ion GeneStudio S5 Prime System?

The server storage size is 25 TB.

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What is the maximum throughput/day for the Ion GeneStudio S5 Plus System?

The Ion GeneStudio S5 Plus System has a maximum throughput/day of 30 Gb (2 Ion 540 Chips).

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How long does it take to run an Ion 550 Chip on the Ion GeneStudio S5 Plus System?

The Ion GeneStudio S5 Plus System takes 11.5 hours to run an Ion 550 Chip.

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How long does it take to run an Ion 540 Chip on the Ion GeneStudio S5 Plus System?

The Ion GeneStudio S5 Plus System takes 10 hours to run an Ion 540 Chip.

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What chips are compatible with the Ion GeneStudio S5 Plus System?

Ion 510 Chip, Ion 520 Chip, Ion 530 Chip, Ion 540 Chip, and Ion 550 Chip are compatible with the Ion GeneStudio S5 Plus System.

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Is the Ion GeneStudio S5 Plus System compatible with the Ion One Touch 2 System?

Yes, the Ion GeneStudio S5 Plus System compatible with the Ion One Touch 2 System.

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Is the Ion GeneStudio S5 Plus System compatible with the Ion Chef System?

Yes, the Ion GeneStudio S5 Plus System compatible with the Ion Chef System.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Does the Ion GeneStudio S5 Plus System have a separate server?

No, the server is contained inside the sequencer itself.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the server storage size on the Ion GeneStudio S5 Plus System?

The server storage size on the Ion GeneStudio S5 Plus System is 24 TB.

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Is the Ion GeneStudio S5 System compatible with the Ion One Touch 2 System?

Yes, the Ion GeneStudio S5 System compatible with the Ion One Touch 2 System.

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Is the Ion GeneStudio S5 System compatible with the Ion Chef System?

Yes, the Ion GeneStudio S5 System compatible with the Ion Chef System.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the server storage size on the Ion GeneStudio S5 System?

The server storage size is 12 TB.

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How long does analysis take on the Ion GeneStudio S5 System?

Please see the following table (https://www.thermofisher.com/us/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-run-sequence/ion-s5-ngs-targeted-sequencing/ion-s5-specifications.html).

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Can the Ion GeneStudio S5 System run two chips back-to-back?

No, the analysis of the first chip must complete before the sequencing run of the second chip begins.

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Does the Ion GeneStudio S5 System have a separate analysis server?

No, the Ion GeneStudio S5 System's analysis server is contained within the sequencer itself.

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How long does it take to run an Ion 540 Chip on the Ion GeneStudio S5 System?

The Ion GeneStudio S5 System takes 19 hours to run and analyze an Ion 540 Chip.

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What is the maximum throughput/day for the Ion GeneStudio S5 System?

The Ion GeneStudio S5 System has a maximum throughput/day of 15 Gb.

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What chips are compatible with the Ion GeneStudio S5 System?

Ion 510 Chip, Ion 520 Chip, Ion 530 Chip, and Ion 540 Chip are compatible with the Ion GeneStudio S5 System.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I perform a templating run with two different types of chips, using any combination of the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip?

Yes, you can perform templating runs with two different types of chips (Ion 510, 520, and 530). However, you cannot mix template sizes (200-base and 400-base template run lengths) in a single run. To perform templating runs with mixed chip types, you must update the Torrent Suite Software to version 5.6 or later.

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What Ion PGM chips are available? How much sequencing data can I expect from each chip type?

The Ion PGM System supports the Ion 3 series chips, including the Ion Torrent Ion 314 Chip Kit v2 (Cat. No. 4482261), Ion Torrent Ion 316 Chip Kit v2 (Cat. Nos. 4483188 and 4483324), and Ion Torrent Ion 318 Chip Kit v2 (Cat. Nos. 4484354 and 4484355). The Ion 314 Chip Kit v2 is available in an 8-pack; the Ion 316 Chip Kit v2 and Ion 318 Chip Kit v2 are available in 4-packs and 8-packs.

For more details, please see the Ion PGM System Specification Sheet (https://tools.thermofisher.com/content/sfs/brochures/PGM-Specification-Sheet.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.