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Ion 540™ Chip Kit
Ion Torrent™

Ion 540™ Chip Kit

The Ion 540™ Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5™ and Ion S5™ XL Sequencing Systems.
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Catalog NumberQuantity
A277654 Chips
A277668 Chips
Catalog number A27765
Price (USD)
4,185.00
Each
Quantity:
4 Chips
Price (USD)
4,185.00
Each

The Ion 540™ Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 540 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of as little as 2.5 hours for 200 bp sequencing.

The Ion 540 Chip Kit offers:

  • Sample tracking functionality from an integrated barcode
  • A rapid run time of as little as 2.5 hours for 200 bp sequencing, generating 60–80 million reads
  • Support for a variety of research applications, including sequencing of up to three human exomes, transcriptome profiling, and targeted resequencing of gene panels
  • Easy handling and loading of templated products for sequencing
  • Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems. Use this kit with the Ion 540 Kit-Chef and the Ion 540 Kit-OT2.

For Research Use Only. Not for use in diagnostic procedures.

Specifications
For Use With (Application)Sequencing
FormatKit
Number of Reads per Chip60-80M
Product TypeChip Kit
Quantity4 Chips
Sequencing TypeGenome and DNA Sequencing
Shipping ConditionRoom Temperature
SpeciesHuman
Product LineIon 540
Unit SizeEach
Contents & Storage
Store at room temperature

Frequently asked questions (FAQs)

Can I use Ion 540 Chip Kit with Ion AmpliSeq Primary Immune Deficiency Research Panel v2? I am worried about the average amplicon length of 243 bp.

Yes, can use Ion 540 chips with an amplicon length of 243 bp. The 200 bp protocol, which is available for all chip sizes including Ion 540 and 550, can be used for amplicons up to 275 bp. Instead, the 400 bp protocol can be used only with Ion 510, 520, and 530 chips with amplicons lengths ranging between 125 and 375 bps.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for the Ion 540 chip?

The Ion 540 chip is shipped at room temperature and should be stored at room temperature.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I reuse the Ion 550 Chef Reagents and Ion 550 Chef Solutions cartridges for running a second chip separately from the first chip?

The Ion 550 Chef Reagents and Ion 550 Chef Solutions cartridges can be reused in a second single-chip templating run if the second run is performed within eight days of the first run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which instrument platforms can I use with the Ion 540 chip?

The Ion 540 chip is compatible with the Ion GeneStudio S5/S5 Plus/Prime and Ion S5/S5 XL instruments.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I perform templating with either the Ion OneTouch 2 System or the Ion Chef System when using the Ion 540 chip for sequencing?

The Ion 540 chip workflow is supported with both the Ion OneTouch 2 System and the Ion Chef System for templating.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

View all Ion 540™ Chip Kit FAQs