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Applied Biosystems™

TaqPath™ 1-Step Multiplex Master Mix

Catalog number: A28525
Applied Biosystems™

TaqPath™ 1-Step Multiplex Master Mix

Catalog number: A28525
Catalog Number
A28525
Unit Size
0.5 mL
Price (USD)
225.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
5 x 1 mL
Price (USD)
2,270.00
Your Price:
Availability
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Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
4,255.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A285250.5 mL
225.00
Your Price:
***
A285265 x 1 mL
2,270.00
Your Price:
***
A2852710 mL
4,255.00
Your Price:
***
Product Overview
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TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics, virus detection, and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE dye (passive reference), and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix is a superior choice for your multiplex diagnostic testing or development needs.

Features of the TaqPath 1-Step Multiplex Master Mix include:
• High sensitivity to detect low-copy targets with reproducible CT results
• Broad dynamic range with both RNA and DNA targets
• Optimized for multiplexing up to four targets simultaneously with MUSTANG PURPLE reference dye
• Tolerant of inhibitors commonly found in clinical samples
• Advanced manufacturing production and process controls insure lot-to-lot consistency
• Compliance Package containing most frequently requested compliance documents, available below

High sensitivity
To enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.

Wide dynamic range compatible with RNA and DNA
TaqPath 1-Step Multiplex Master Mix has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.

Optimized for multiplexing
TaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. Rather than including ROX dye as a passive reference, the TaqPath 1-step Multiplex Master Mix includes the longer emission wavelength MUSTANG PURPLE dye as a passive reference. This longer wavelength passive reference dye enables measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix performance in 4-plex reactions with both RNA and cDNA targets.

Inhibitor tolerant
Unlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).

Manufacturing production and process controls
TaqPath 1-Step Multiplex Master Mix is manufactured under a strong quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot.

General Purpose Reagent manufactured under cGMP
TaqPath master mixes are general purpose reagents labeled “For Laboratory Use” and manufactured in an ISO 13485-certified and FDA-registered facility that adheres to cGMP principles.

Compliance Package
In an effort to efficiently support your compliance needs, frequently requested compliance documents are available here:
Statement of Quality relating to the current good manufacturing practices (cGMP) to which TaqPath master mixes adhere
• Detailed lot-specific Certificate of Analysis (COA) (see below in Documents & Downloads section)
Product Stability Statement
• Supplier Evaluation Support
   -- Completed Self Assessment
   -- ISO certificates by manufacturing site
• For EU Reach compliance-related inquiries, email "LSGChemicalCompliance@thermofisher.com"
• BSE/TSE Statement
• Change Control and Notification Registration
Not finding the compliance document you’re looking for? Technical Support is here to help.

Specifications

Concentration
4X
Detection Method
Primer-Probe Detection
Format
Tube
No. of Reactions
100 Reactions
PCR Method
1-step RT-qPCR, Multiplex qPCR
Polymerase
Fast DNA Polymerase
Reaction Speed
Fast or Standard
Sample Type
DNA, RNA
Volume
0.5 mL
For Use With (Application)
Real Time PCR (qPCR)
For Use With (Equipment)
7500 Fast System, 7500 System, QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, QuantStudio™ Dx, 7500 Fast Dx System
Product Line
TaqPath™
Product Type
qPCR Master Mix
Quantity
0.5 mL
Shipping Condition
Dry Ice
Sufficient For
100 Reactions of 20 μL

Contents & Storage

Contains one 0.5-mL tube of 4 x RT-qPCR master mix sufficient for 100 20-µL reactions.
Store in freezer (-5°C to -30°C).

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