Applied Biosystems™

TaqPath™ 1-Step Multiplex Master Mix

Catalog number:  A28527

 Related applications:

Real Time PCR (qPCR)

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Description

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics, virus detection, and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE dye (passive reference), and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

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This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix is a superior choice for your multiplex diagnostic testing or development needs.

Features of the TaqPath 1-Step Multiplex Master Mix include:
• High sensitivity to detect low-copy targets with reproducible CT results
• Broad dynamic range with both RNA and DNA targets
• Optimized for multiplexing up to four targets simultaneously with MUSTANG PURPLE reference dye
• Tolerant of inhibitors commonly found in clinical samples
• Advanced manufacturing production and process controls insure lot-to-lot consistency

High sensitivity
To enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.

Wide dynamic range compatible with RNA and DNA
TaqPath 1-Step Multiplex Master Mix has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.

Optimized for multiplexing
TaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. Rather than including ROX dye as a passive reference, the TaqPath 1-step Multiplex Master Mix includes the longer emission wavelength MUSTANG PURPLE dye as a passive reference. This longer wavelength passive reference dye enables measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix performance in 4-plex reactions with both RNA and cDNA targets.

Inhibitor tolerant
Unlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).

Manufacturing production and process controls
TaqPath 1-Step Multiplex Master Mix is manufactured under a strong ISO13485 quality management system that uses traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot. Figure 5 demonstrates CT lot-to-lot consistency is preserved across multiple assays with different attributes and input target levels.

Broad instrument compatibility
The TaqPath 1-Step Multiplex Master Mix can be run in either fast or standard cycling conditions with equivalent performance across a wide variety of real-time PCR systems including the Applied Biosystems 7500, 7500 Fast, 7500 Fast Dx, QuantStudio 3, 5, 6, 7, 12k, Dx, and ViiA 7 real-time PCR systems.

General purpose reagent
TaqPath 1-Step RT-qPCR Master Mix is a General Purpose Reagent (GPR), labeled for laboratory use.

Related products
TaqPath 1-Step Master Mix is available in three different formulations:
TaqPath 1-Step qRT-PCR Master Mix—includes ROX dye as the passive reference
TaqPath 1-Step Multiplex Master Mix (this page)—includes MUSTANG PURPLE dye as the passive reference
TaqPath 1-Step Multiplex Master Mix (no ROX)—does not include a passive reference dye

For Laboratory Use.

Specifications

PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Fast or Standard
For Use With (Equipment): 7500 Fast Dx System, 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx, ViiA™ 7 System
Sample Type (General): DNA, RNA
Product Line: TaqPath™
Product Size: 10 mL
Shipping Condition: Dry Ice

Contents & storage

Contains one 10-mL tube of 4X RT-qPCR master mix sufficient for 2000 20-µL reactions.
Store in freezer (-5 to -30°C).

Documents

Manuals & protocols