Attune™ NxT Flow Cytometer, blue/red/yellow, refurbished
Invitrogen™

Attune™ NxT Flow Cytometer, blue/red/yellow, refurbished

This is a refurbished Attune NxT Flow Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity,Read more
Have Questions?
Catalog NumberQuantity
A28993R1 cytometer
Catalog number A28993R
Price (EUR)
-
Quantity:
1 cytometer
This is a refurbished Attune NxT Flow Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity, as well as fluorescence expression of labeled antibodies, proteins, and functional reagents. The innovative design of the instrument results in precise data and acquisition speeds up to 10X faster than other flow cytometers. The design also eliminates lengthy sample preparation procedures, reduces the time to analyze a file, allows small or limited samples to be analyzed, and minimizes variability in measurements, leading to better resolution of populations. It is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, and microbiological assays.

With the Attune NxT Flow Cytometer you get:
• A flexible platform that provides highly sensitive and accurate measurement of cell sizeand granularity, as well as fluorescence detection
• State of the art optics for long-term reliability
• Acoustic focusing for high-precision data
• An intuitive software user interface that reduces time to proficiency
• Improved workflow to reduce sample preparation time before analysis
• Faster data analysis that leads to reduced acquisition times

Flexible design that accommodates changing needs, budget, or laboratory space
The instrument's modular design accommodates the most common fluorophores and fluorescent proteins used in flow cytometry enabling alignment with previous experimental results and accommodating future needs through simple upgrades. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.

Acoustic focusing improves efficiencies compared to traditional flow cytometers
Traditional flow cytometers rely on a large volume of fluid to focus cells prior to analysis, resulting in extensive sample preparation prior to analysis and introducing the possibility of error due to artifacts created during the sample preparation process. With the Attune NxT Flow Cytometer, blood samples can be run without being manipulated using the No-Wash No-Lyse blood cell assay. The instrument also offers flexibility in the sample concentration that can be analyzed.

Acoustic focusing enables:
• Elimination of concentration steps
• Effective background fluorescence reduction by a simple dilution step, eliminating the need to wash fluorescence samples before analysis
• No-lyse/no-wash assays
• Analysis of samples that have low cell numbers, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
• Acquisition of a high volume of samples in minimal time

Learn more about the Attune NxT cytometer and see all available configurations >
Learn more about how acoustic focusing works >
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatTube(s), 384-well plate, 96-well plate
Laser FeaturesBlue Excitation Laser (488 nm), Red Excitation Laser (638 nm), Yellow Excitation Laser (561 nm)
Product LineAttofluor
Quantity1 cytometer
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Unit SizeEach
Contents & Storage
• Attune Acoustic Focusing Cytometer, refurbished
• Minitower Windows™ 7 64-bit computer with 23-inch flat panel monitor
• Power cord with adapters
• USB cable
• Attune NxT software
• Quick reference card
• 1 vial of Attune Performance Tracking Beads
• 1 bottle of Attune Wash Solution
• 1 bottle of Attune 1X Shutdown Solution
• Attune Focusing Fluid

Frequently asked questions (FAQs)

My Attune NxT Flow Cytometer performance test keeps failing. What steps can I take to minimize the number of failed performance tests?

To minimize the number of failed performance tests on your Attune NxT Flow Cytometer, we suggest the following:

•   Make sure to carry out daily, weekly, and monthly maintenance procedures (pages 8-14 of the Maintenance and Troubleshooting Guide, linked below).
•   Always run 3 startup cycles and 2 Rinse procedures (pages 46-47 of the Maintenance and Troubleshooting Guide linked below).
•   If the performance test fails, carry out the following priming sequence: run 3 startup cycles, 2 de-bubble cycles, and 5 rinse procedures, followed by running the SIP sanitize function.

Please review the Attune Flow Cytometry Maintenance and Troubleshooting Guide for more information about performing routine maintenance procedures and troubleshooting.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

Can I use the Attune Nxt Flow Cytometer to detect bacteria?

Yes. The Attune Nxt Flow Cytometer can detect down to 0.2 µm and using a special configuration, down to 0.1 µm. Optimize your settings using Flow Cytometry Sub-micron Particle Size Reference Kit, Cat. No. F13839.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Of all the Attune NxT filters you offer, I don't see one that matches our application. Can I use a filter other than what you offer?

Yes. You would need to purchase Attune NxT Custom Filter Holder Kit, Cat. No. A27784 to make your own custom emission or dichroic filters in order to customize the optical configuration of the instrument. Each kit contains sufficient parts to make 2 emission filters and 2 dichroic filters. The kit includes instructions for attaching the filter to the filter blade, but does not include the glass filter. Other accessories to optimize for special applications include Cat No. 100083194 Attune NxT Small Particle Side-Scatter Filter, Cat. No. 100083194 and Attune NxT Blocker Bar Conversion Kit, Cat No. A35966.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the Attune Nxt Flow Cytometer, if I wish to only start with a single laser, can I expand to more lasers and channels?

Yes. There are 6 lasers to choose from (blue, green, yellow, red, violet, and violet 6). You can start with one laser and at any time upgrade the same instrument with up to 4 lasers to utilize a maximum of 14 detection channels. Using the Violet 6 laser along with the blue, red, and yellow lasers, one can detect up to 16 channels.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What makes the Attune Nxt Flow Cytometer better than standard flow cytometers?

Because of the acoustic-assisted hydrodynamic technology, the Attune NxT Flow Cytometer uses only 1/10th the amount of fluids, and is 10X faster and less sensitive to clogging compared to standard flow cytometers. You can set the flow rate from 12.5-1,000 µL/min and collect up to 65,000 events/sec using from 20 µL to 4 mL of sample volume. These unique properties allow for rare event analysis, as well standard flow analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.