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Gibco™

ExpiCHO™ Expression Medium

Catalog number: A2910001
Have Questions?
Gibco™

ExpiCHO™ Expression Medium

Catalog number: A2910001
Have Questions?
Catalog Number
A2910001
Unit Size
1000 mL
Price (USD)
Availability
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Catalog Number
Unit Size
6 x 1 L
Price (USD)
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Quantity
Catalog Number
Unit Size
20 L
Price (USD)
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-
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Catalog Number
Unit Size
10 L
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Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A29100011000 mL
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A29100026 x 1 L
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A291000420 L
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A291000310 L
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ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium:
• Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
• Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent
• Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
• Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
• Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors
• Does not contain phenol red

ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.

Specifications

Cell Line
CHO-S, SFM Adapted
Classification
Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Culture Type
Suspension Cell Culture
Form
Liquid
Product Type
CHO Expression Medium
Serum Level
Serum-free
With Additives
GlutaMAX
Without Additives
No Phenol Red
Format
Bottle
Green Features
Sustainable packaging
Product Line
ExpiCHO™
Quantity
1000 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: in the dark at 2-8°C
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture

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