Informational:cGMP-banked ExpiCHO-S cells now available. Click HERE for product information.
Gibco™

ExpiCHO-S™ Cells

Catalog number: A29127
Have Questions?
Gibco™

ExpiCHO-S™ Cells

Catalog number: A29127
Have Questions?
Catalog Number
A29127
Unit Size
1 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A291271 mL
-
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ExpiCHO-S™ Cells are derived from a non-engineered subclone that has been screened and isolated from CHO-S Chinese hamster ovary (CHO) cells (1). They are a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). ExpiCHO-S Cells are maintained in suspension culture, have minimal tendency to clump, and grow to high density in ExpiCHO™ Expression Medium (Cat. No. A2910001). As part of the ExpiCHO Expression System Kit, the cells generate superior protein yields by transient transfection compared to standard CHO, 293, and high-density Expi293F™ cells.

ExpiCHO-S™ Cells:

  • Allow you to attain yields of up to 3 g of protein per L of transfected culture as part of the ExpiCHO expression system
  • Enable rapid, high-yield transient protein production, allowing labs developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
  • Are adapted to high-density suspension growth in ExpiCHO Expression Medium
  • Exhibit rapid doubling times of approximately 17 hours
  • Enable consistent transient expression performance for 20 passages after thaw
  • Maintain high cell viability and productivity for greater than 10 days post-transfection as part of the ExpiCHO expression system
  • Also available from a cGMP bank (Cat. No. A37785)

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells per mL in ExpiCHO Expression Medium and 10% DMSO. The cells should be thawed directly into ExpiCHO Expression Medium. The 6-vial multipack eliminates the need to expand and bank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of 10 L in bioreactors. Protein harvest can be performed at 5 to 14 days post-transfection, depending on the protein expressed.

References

1. Wurm, FM. CHO Quasispecies—Implications for Manufacturing Processes (2013) Processes 1:296-311.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line
CHO-S, SFM Adapted
Culture Type
Suspension Cell Culture
Form
Cryopreserved
Species
Hamster
Format
1 vial
For Use With (Application)
Expression
No. of Cells
1 x 10^7 cells
Product Line
ExpiCHO™
Product Type
CHO-S Cell
Shipping Condition
Dry Ice

Contents & Storage

1 x 107 cells/mL

Store in liquid nitrogen.

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