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Gibco™

ExpiFectamine™ CHO Transfection Kit

Catalog number:  A29131

 Related applications:

Protein Expression

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Description

The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).

The ExpiFectamine CHO Transfection Kit:
• Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
• Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
• Provides options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
• Enables robust and reproducible transfection results, giving you greater confidence in your results
• Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Established Cell Lines, Ovarian Cells
Sample Type (Specific): Chemical Solution
Classification: Animal Origin-Free, Chemically Defined
Transfection Technique: Lipid-Based Transfection
Product Line: ExpiFectamine™
Product Size: For 50 L of culture
Shipping Condition: Room Temperature

Contents & storage

Five 10-L kits; sufficient for transfection of 50 L of culture

160 mL ExpiFectamine CHO Reagent
300 mL ExpiFectamine CHO Enhancer
16.5 L ExpiCHO Feed

Store in the dark at 2 -8°C.

Documents

Manuals & protocols