ExpiCHO-S™ Cells

Features of ExpiCHO-S Cells include:

• Suspension culture cells adapted in ExpiCHO Expression Medium
• Demonstrated yields of up to 3 g of protein per L of transfected culture as part of the ExpiCHO expression system
• Rapid doubling times of approximately 17 hours
• Consistent transient expression performance for over 20 passages after thaw
• High cell viability and productivity for greater than 10 days post-transfection as part of the ExpiCHO expression system
• Enable rapid, high-yield transient protein production, allowing labs developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells per mL in ExpiCHO Expression Medium and 10% DMSO. The cells should be thawed directly into ExpiCHO Expression Medium. The 6-vial multipack eliminates the need to expand and bank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO₂ cell culture incubator. However, cultures are scalable from volumes of <1 mL up to 5 L when using a Nalgene 5-L angled-bottom flask. Protein harvest can be performed at 5 to 14 days post-transfection, depending on the protein expressed.

Reference

1. Wurm, FM. CHO Quasispecies—Implications for Manufacturing Processes (2013) Processes 1:296-311.