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Gibco™

ExpiCHO™ Expression System Kit

Catalog number:  A29133

 Related applications: Protein Expression

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Description

The ExpiCHO™ Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S™ cells in ExpiCHO™ Expression Medium. Transient expression is powered by ExpiFectamine™ CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed. All components work together synergistically to generate 2- to 10-fold higher protein yields than the HEK293-based Expi293™ Expression System Kit and 20- to 150-fold higher yields than the FreeStyle™ MAX CHO Expression System. Expression yields of 1 to 3 g per L of transfected culture have been demonstrated for some antibody and non-antibody proteins.

The ExpiCHO Expression System offers:

• Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently higher than transient 293-based systems
• Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
• A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells
• Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
• Scalable expression for cultures from <1 mL to >10 L, thus generating the amount of protein needed for your application

The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO™ SFM, and an antibody-expressing positive control vector. All components of the ExpiCHO Expression System Kit are also available for purchase separately. All components of the system are animal origin–free.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: CHO-S, SFM Adapted
Delivery Method: Transfection
Key Function: Constitutive Expression, Protein Production
Product Line: ExpiCHO™
Product Size: 1 kit
Promoter: CMV
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

• 2 vials frozen ExpiCHO-S cells
• 1000 mL ExpiCHO Expression Medium
• 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
• 1 vial Antibody Expressing Positive Control Vector
• 100 mL OptiPRO SFM

Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.

Documents

Manuals & protocols