For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
Invitrogen™

PureLink™ Microbiome DNA Purification Kit

Catalog number:  A29790

 Related applications:

DNA Extraction

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Description

The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. The kit uses proven PureLink spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing, or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility make this the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

Features of the PureLink Microbiome DNA Purification Kit include:

• Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
• Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
• Streamlined protocols for numerous biological samples
• Recovery of high-purity DNA compatible with common downstream applications such as qPCR and next-generation sequencing

One kit to isolate microbial and host DNA from a diverse set of sample types
The PureLink Microbiome DNA Purification Kit minimizes the need to order individual kits for specific sample types because it has been optimized for use with a wide range of biological samples.

The kit enables microbial—and, where applicable, host—DNA purification from the following samples:

• Stool
• Urine
• Saliva
• Swabs (vaginal, buccal, skin, rectal, environmental)
• Transport media
• Growth media
• Soil

Analyzing the human microbiome
The human body is populated by 100 trillion bacteria, archaea, fungi, protists, and viruses, all of which play a fundamental role in our well-being. The term "microbiome" refers to the various different microbes in those communities. Presently, their genes are the most straightforward method of identifying these organisms. Deviations from healthy microbial compositions have been linked to many human diseases, including inflammatory bowel disease, obesity, cancer, asthma, diabetes, and allergies.

Purification of the aggregate microbial DNA in a biological sample is a key step in understanding how the microbiome influences human health. It is of utmost importance that the DNA recovered accurately reflects the representation of the diverse microbes in the sample community. The mechanical disruption and triple lysis approach used in the PureLink Microbiome DNA Kit help ensure that microbial DNA from even the toughest samples, such as gram-positive bacteria found in stool, can be recovered—allowing you to identify the full spectrum of microbes present.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria, Buccal Samples, Cells, Food & Environmental Samples
High Throughput Compatibility: Not High Throughput-Compatible
Downstream Application: Next-Generation Sequencing, PCR, Real-Time PCR
Product Line: PureLink™
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

• S1-Lysis Buffer, 1 x 40 mL
• S2-Lysis Enhancer, 1 x 5 mL
• S3-Cleanup Buffer, 1 x 12.5 mL
• S4-Binding Buffer, 1 x 45 mL
• S5-Wash Buffer Concentrate, 1 x 13 mL
• S6-Elution Buffer, 1 x 5 mL
• PureLink Spin Columns with Collection Tubes, 50 columns/tubes
• PureLink Collection Tubes, 100 tubes
• Bead Tubes*, 50 tubes containing specialized beads
*Ships in a separate box.

Store at room temperature.

Documents

Manuals & protocols