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Ion 540™ Kit-Chef (2 sequencing runs per initialization)
Ion Torrent™

Ion 540™ Kit-Chef (2 sequencing runs per initialization)

The Ion 540™ Kit-Chef enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef™ and the Ion S5™ or Ion S5™ XL Sequencing Systems.
Have Questions?
Catalog NumberQuantity
A300118 reactions
Catalog number A30011
Price (USD)
10,140.00
Each
Quantity:
8 reactions
Price (USD)
10,140.00
Each

The Ion 540™ Kit-Chef enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef™ and the Ion S5™ or Ion S5™ XL Sequencing Systems. The Ion 540 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. The kit contains sufficient reagents and supplies for 4 initializations and 8 sequencing runs on the Ion S5 or Ion S5 XL systems.

The Ion 540 Kit for the Ion Chef and Ion S5/Ion S5 XL workflow:

  • Provides template preparation and chip loading reagents for up to 200 base-read libraries
  • Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
  • Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
  • Leverages the latest advancements in data quality and accuracy

The Ion S5/Ion S5 XL sequencers, along with the Ion OneTouch™ 2 System, provide reproducible sequencing workflows. Delivering up to 80 million reads in a single run, the Ion 540 Chip empowers rapid exome and transcriptome analysis. Combined with Ion AmpliSeq™ technology and Ion Reporter™ Software for data interpretation, the Ion S5/Ion S5 XL systems provide a cost-effective and comprehensive solution for your translational or inherited disease research needs.

For Research Use Only. Not for use in diagnostic procedures.

Specifications
Product TypeChef Kit
Quantity8 reactions
Shipping ConditionQualified for Ambient or Wet Ice
Workflow StepTemplate Preparation
Product LineIon 540
Unit SizeEach
Contents & Storage
• Ion S5 Chef Solutions, store at room temperature
• Ion S5 Chef Supplies, store at room temperature
• Ion 540 Chef Reagents, store at -5°C to -30°C
• Ion S5 Sequencing Solutions, store at room temperature
• Ion S5 Sequencing Reagents, store at -5°C to -30°C

Frequently asked questions (FAQs)

Which Ion Chef kit should I use for 200 bp vs 400 bp sequencing workflows?

The Ion 540 Kit - Chef kit is designed for automated template preparation and sequencing of libraries up to 200 bp. For 400 bp library workflows, use the Ion 530 Kit - Chef kit (Cat. No. A34019), which supports longer read lengths.

Is Ion S5 Sequencing Reagents (Part No. A27768) used for both the Ion 540 and Ion 550 Chip sequencing?

Yes, Ion S5 Sequencing Reagents (Part No. A27768) is used on the instrument for both Ion 540 and Ion 550 Chip sequencing.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Microbiome Health Research Kit, Library bundle?

The Ion AmpliSeq Microbiome Health Research Kit, Library bundle (Cat. No. A46495) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, and the IonCode Barcode Adapters for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 48 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use the AmpliSeq_Microbiome_Health_ Analysis plugin for Torrent Suite Software and Ion Reporter Software workflows for my custom 16S or target species AmpliSeq panel?

The Torrent Suite AmpliSeq_Microbiome_Health_ Analysis plugin and Ion Reporter Software workflows were developed and validated exclusively for the Ion AmpliSeq Microbiome Health Research Kit. Open source analysis tools may be available for your custom solutions.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

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