Alexa Fluor™ Plus 647 Phalloidin

Catalog number: A30107

Description

Alexa Fluor Plus 647 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, Cy5/deep red fluorescent Alexa Fluor Plus 647 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 647 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 647 Phalloidin per length of F-actin.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidin for F-actin staining ›

• Selectively stains F-actin with 2–3 times the sensitivity of Alexa Fluor 647 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 650/668 nm. Compatible with standard Cy5/deep red/far red filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides "death cap" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

For Research Use Only. Not for use in diagnostic procedures.

Figures

HeLa cells labeled with Alexa Fluor Plus 647 Phalloidin

Mouse kidney labeled with Alexa Fluor Plus 647 Phalloidin

HeLa cells labeled with Alexa Fluor Plus 647 Phalloidin

Multiple cell lines stained with Alexa Fluor Plus 647 Phalloidin

Alexa Fluor Plus 647 Phalloidin fluorescence excitation/emission spectra

Specifications

Excitation⁄Emission (nm):	650⁄668
Label or Dye:	Alexa Fluor Plus 647
Product Line:	Alexa Fluor™
Product Size:	1 each
Shipping Condition:	Qualified for Ambient or Wet Ice
Sub-Cellular Localization:	Actin

Contents & storage

Contains 1 vial with enough material for 300 units/slides.

Store at ≤–20°C after receiving. Desiccate and protect from light.
When stored as directed, product is stable for at least 1 year.