Applied Biosystems™

TaqPath™ ProAmp™ Master Mix

Catalog number:  A30866

 Related applications:

Real Time PCR (qPCR)

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TaqPath™ ProAmp™ Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mixes are designed to deliver sensitive and reproducible results from genomic DNA targets. The simple genotyping and copy number variation workflow and reproducible performance even in the presence of inhibitors provides confidence in results.

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Two TaqPath ProAmp Master Mix formulations for different throughput needs

• TaqPath ProAmp Master Mix—includes ROX™ passive reference dye for lower multiplexing of up to 3 targets
• TaqPath ProAmp Multiplex Master Mix—includes MUSTANG PURPLE™ passive reference dye for higher multiplexing of up to 4 targets

Benefits of TaqPath ProAmp Master Mixes

Exceptional data quality—high specificity, dynamic range*, and reproducibility for genotyping and copy number determination even in the presence of inhibitors
Tolerance of inhibitors common in samples prepared from human or animal sources (buccal swabs, blood, and card punches)
72-hour pre-PCR benchtop stability for automated workflows, enabled by our proprietary Dual-Lock™ Taq DNA hot-start mechanism
Multiplexing breadth—two formulations that enable single to four-target detection per reaction
Excellent manufacturing consistency—manufactured in an ISO 13485-certified facility

High accuracy genotyping with purified or crude lysate sample inputs
Accurate genotyping is important when limited sample is available or PCR inhibitors are unavoidable, such as in clinical diagnostic testing. TaqPath ProAmp Master Mixes have been optimized to yield accurate and reproducible results, even in low-volume reactions prepared with purified or crude lysate sample inputs. Figure 1 shows a comparison of genotyping accuracy versus competitor mixes for the same set of TaqMan Genotyping Assays using crude lysate from blood samples. TaqPath ProAmp Master Mixes have been optimized to provide high-specificity allelic discrimination as demonstrated by excellent cluster separation in genotyping plots, whether the sample is purified genomic DNA or crude lysate.

High accuracy copy number quantitation
The versatile TaqPath ProAmp Master Mixes can also be used for highly accurate determination of copy number variation using purified genomic DNA samples. Figure 2 shows a comparison of copy number variation results using TaqPath ProAmp Master Mix versus two other mixes. TaqPath ProAmp master mix enables determination of copy number accurately in samples with up to 4 copies.

General purpose reagents
TaqPath master mixes are general purpose reagents manufactured in an ISO 13485-certified facility and labeled "For Laboratory Use."

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects >

* Dynamic range is a property of both the assay and template concentration in the sample, as well as the formulation of the master mix; thus, individual results may vary.


Detection Method: Primer-Probe Detection
For Use With (Equipment): 7500 Fast Dx System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 12k Flex Dx, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx, StepOne™ System, StepOnePlus™ System, ViiA™ 7 System
Reaction Speed: Standard & Fast Mode
PCR Method: qPCR
Product Line: TaqPath™
Product Size: 1 x 10 mL
Shipping Condition: Wet Ice

Contents & storage

Store at 2–8°C.


Manuals & protocols