Ion AmpliSeq™ Transcriptome Human Gene Expression Panel, Chef-Ready Kit

The Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit, enables automated production of Ion Ampliseq transcriptome libraries on the Ion Chef System. For manual library production, see the Ion AmpliSeq Transcriptome Human Gene Expression Kit.

The Ion AmpliSeq Transcriptome Human Gene Expression Panel allows simultaneous gene expression measurement of over 20,000 human RefSeq genes in a single assay. The Chef-ready kit utilizes the same chemistry as that designed for a manual workflow, providing equivalent or better performance with exceptional reproducibility, on-target reads, and sample balance. Libraries can be produced from as little as 10 ng of total RNA from FFPE or fresh/frozen samples with only 15 minutes of hands-on time to setup the Ion Chef System.

In addition, the Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit, offers:
• Wider dynamic range and improved detection sensitivity compared to conventional microarray analysis
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting, as well as quality control metrics
• Compatibility with Ion Xpress barcode adapters allowing for multiplex sequencing

Wider dynamic range for enhanced performance
With a wider dynamic range and improved gene detection compared to conventional microarray analysis, the Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-ready Kit, offers a clear solution to researchers needing enhanced performance for their gene expression needs. A simple workflow allows for sample-to-gene quantitation in less than 2 days, helping to reduce workload and increase throughput.

Straightforward library preparation and multiplex sequencing
The Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit, includes the reagents and materials required for automated preparation of 32 barcoded Ion AmpliSeq transcriptome libraries. The Ion Chef System can automate up to 8 barcoded libraries per run, so the kit provides reagents and materials sufficient for 4 runs. Starting with as little as 10 ng of total RNA from FFPE or fresh/frozen samples, the total RNA is first processed using the SuperScript VILO cDNA Synthesis Kit (not included), and the resulting cDNA is amplified using Ion AmpliSeq technology to accurately maintain expression levels of all genes. Approximately 7 hours of instrument run time and only 15 minutes of hands-on time are needed to set up the Ion Chef System for library construction from cDNA.

The resulting libraries are ready for downstream template preparation using the Ion Chef System and sequencing on the Ion GeneStudio S5 Sequencing System. With the recommended 16 libraries per Ion 550 Chip and 8 libraries per Ion 540 chip, libraries are multiplexed for flexibility, throughput increase, and cost reduction. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.

Automatic data analysis with Torrent Suite plug-in
Using the complementary analysis plug-in available with Torrent Suite software, gene quantitation information is automatically compiled, minimizing the requirement for costly computational resources and bioinformatics expertise to process raw sequencing data into meaningful information. An easy-to-interpret, highly portable table is produced containing all genes interrogated and their respective expression levels—output that may be used with a variety of existing software applications for downstream analysis of microarray or next-generation sequencing data.

Learn more about the Ion AmpliSeq Transcriptome Human Gene Expression Kit ›