Suero fetal bovino, cualificado, Nueva Zelanda
Suero fetal bovino, cualificado, Nueva Zelanda
Gibco™

Suero fetal bovino, cualificado, Nueva Zelanda

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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
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Número de catálogoCantidadFormato
A316090210 x 50 mLOne Shot
A316090150 mLOne Shot
10091148500 mLFrasco
Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Número de catálogo A3160902
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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Categoría de los sueros: Premium (Secure)
Origen : Nueva Zelanda
Nivel de endotoxinas: ≤5 EU/ml.
Nivel de hemoglobina: ≤30 mg/dl (niveles habituales ≤25 mg/dl).
Para uso diagnóstico in vitro.
Especificaciones
DescripciónSuero bovino fetal, cualificado, formato One Shot™, Nueva Zelanda
Concentración de endotoxinas≤5 EU/mL
Concentración de hemoglobina≤30 mg/dL
Línea de productosOne Shot
Grado de pureza o calidadCualificado
Condiciones de envíoCongelado
EspecieBovino
AgeFetal
País de origenNueva Zelanda
FormularioLíquido
FormatoOne Shot
Tipo de productoSuero fetal bovino
Cantidad10 x 50 mL
Serum TreatmentEstándar (esterilizado mediante filtro)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

Why would I heat inactivate serum?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat inactivated serum is recommended include immunological studies and culturing ES cells, insect cells, and smooth muscle cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I heat inactivate serum?

Heat at 56 degrees C in a water bath for 30 minutes. Swirl bottle every 10 minutes or so. For accuracy, use a second bottle of similar size and add the equivalent volume of water to this bottle. Place a thermometer in this control bottle in order to see when 56 degrees C is reached. Set your timer for 30 minutes at this point.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

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