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Gibco™

Vitronectin (VTN-N) Recombinant Human Protein, Truncated

Catalog number:  A31804

Related applications:

Stem Cell Culture

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Description

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium (A1517001), vitronectin (VTN-N) has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines. In addition, vitronectin (VTN-N) has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. As published by Guokai Chen et al. (1) in the laboratory of James Thomson, the VTN-N variant of vitronectin supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8™ Medium.

Vitronectin (VTN-N) is:

Optimized—designed in the laboratory of James Thomson for use with Essential 8™ Medium
Defined—for reduced variability in your PSC cultures
Cost effective—for economical and scalable PSC culture

Optimized and Defined Surface
Unlike standard basement membrane extracts (BMEs), vitronectin (VTN-N) was designed in the laboratory of James Thomson for use with Essential 8™ Medium and has been shown to support human pluripotent stem cell attachment and survival better than wild type vitronectin. Vitronectin (VTN-N) provides an optimal environment for feeder-free pluripotent stem cell culture.

Reduce Variability
Vitronectin (VTN-N) is a defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs).

Cost Effective
When used with Essential 8™ Medium, vitronectin (VTN-N) provides a cost effective, defined system for feeder free culture of human pluripotent stem cells (PSCs).

Commercialized in Partnership with Cellular Dynamics International.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration: 500 µg⁄ml
Expression: E. coli
Product Size: 10 mL
Protein Form: Recombinant,Truncated
Protein Subtype: Vitronectin
Purity or Quality Grade: Cell Culture Grade,Endotoxin-Screened
Research Category: Stem Cell Research
Shipping Condition: Dry Ice
Species: Human

Contents & storage

Store at -80°C and protect from light.