NOTE: Gibco KnockOut Serum Replacement—Multi-Species will be discontinued as of January 31, 2021. Consider KnockOut Serum Replacement (10828028) for a seamless transition.

KnockOut™ Serum Replacement - Multi-Species This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  A3181502

Related applications:

Stem Cell Culture

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Gibco™ KnockOut™ Serum Replacement – Multi-Species (KnockOut SR – Multi-Species) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this Research Use Only product uses the same KnockOut Serum Replacement formulation that has been trusted by many for almost 20 years. For pre-clinical or clinical applications, please see Cat. No. 10828028.

With KnockOut Serum Replacement – Multi-Species, you can:
• Culture mouse, human, and other mammalian feeder-dependent PSCs with confidence
• Rely on the improved performance of a qualified, FBS-free formulation
• Enjoy the peace of mind of having a more stable price and supply compared to FBS

Culture mammalian PSCs with confidence
The KnockOut SR – Multi-Species formulation has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from mouse, human, and other mammalian species. For mouse and human PSCs, it has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

Rely on improved performance
KnockOut SR – Multi-Species has a more defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing mouse and human PSCs with KnockOut SR – Multi-Species leads to less differentiation and more compact colony morphology compared to FBS.

Enjoy the peace of mind of having stable price and supply
KnockOut SR – Multi-Species is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.

For Research Use Only. Not for use in diagnostic procedures.


Classification: Serum-Free
Green Features: Sustainable packaging
Manufacturing Quality: cGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product Line: KnockOut™
Shipping Condition: Dry Ice
Quantity: 500mL

Contents & storage

Store in freezer (-5 to -20°C) and protect from light.