The Tag Sequencing Barcode Set 1-24 provides a set of 24 unique barcode adapters specifically designed for optimal performance inRead more
Have Questions?
Catalog Number
Quantity
A31830
1 set
Catalog number A31830
Price (USD)
1,520.00
Each
Add to cart
Quantity:
1 set
Price (USD)
1,520.00
Each
Add to cart
The Tag Sequencing Barcode Set 1-24 provides a set of 24 unique barcode adapters specifically designed for optimal performance in Oncomine™ cfDNA assays. The barcode adapters are compatible with the Ion S5™, Ion PGM™, and Ion Proton™ sequencing systems. When used in combination with kits utilizing tag sequencing technology, this barcode set enables users to pool up to 24 amplicon libraries and to conduct multiplexed next-generation sequencing (NGS) analysis, reducing the sequencing cost per sample.
Key product features: • Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes • Both sequence-optimized and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs • Compatible with library construction kits utilizing tag sequencing technology
Multiplexing with barcode adapters enables higher throughput Multiplexing with barcoded libraries leads to more cost-effective runs by permitting up to 24 samples per run (or more if combined with Tag Sequencing BC Set 25-48, Cat. No. A31847), significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias.
Sequence-optimized and flow-optimized for increased performance and efficiency Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool; they require a minimal number of flows to interrogate resulting in more economical multiplexed sequencing runs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Next-Generation Sequencing (NGS)
FormLiquid
No. of Reactions10
PrimerCustom Primer
Quantity1 set
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
10 μL each Tag Sequencing Barcodes 01 thru 24, 10 reactions/barcode
Store in freezer (-5°C to -30°C).
Frequently asked questions (FAQs)
What are the differences between the Tag Sequencing Barcode Set 1-24 (Cat. No. A31830) and the Ion Xpress Barcode Adapters (Cat. No. 4474517)?
The TAG Sequencing Barcode Set contains adapters designed for Oncomine workflows using AmpliSeq HD technology and they can be used only with those library kits. This type of chemistry uses a molecular tag in order to track polymerase errors and increase the assay sensitivity. The Ion Xpress Barcode Adapters are not an alternative of the TAG Sequencing Barcode Set and vice versa. The list of the barcodes set compatible with a library kit is specified in the library manual.
For the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay, what are the analysis workflows in Ion Reporter Software?
There are two analysis workflows available:
1. Oncomine TagSeq Lung v2 Liquid Biopsy - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.1% limit of detection), Fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell‑Free Total Nucleic Acid Research Assay. This is compatible with DNA & RNA purified from cell-free total nucleic acids.
2. Oncomine TagSeq Lung v2 Tumor - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.5% limit of detection), fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Due to deamination events caused by the FFPE process, the minimum alternative allele frequency is set to 0.3%. This makes it compatible with DNA & RNA purified from FFPE tumor tissue as well as fresh frozen tumor tissue.
Are there any BED files for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay?
Yes, there is a specific BED file and Hotspot file for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Please contact your local Field Application Specialist (FAS) or Clinical Application Consultant (CAC) to request the BED files.
What is the LOD (limit of detection) for the Oncomine Lung Cell-Free Total Nucleic Acid (cfNA) Research Assay?
Through the use of Tag Sequencing technology, low limits of detection (LOD) can be achieved for different variant types*:
- For SNVs/short indels, an LOD of 0.1% can be achieved with sensitivity of 90% and specificity of >99%
- For fusions & MET exon skipping, an LOD of 1% can be achieved with sensitivity of >90% and specificity of >99%
- For MET CNV target, detection as low as 1.2-fold amplification can be achieved with sensitivity of >90% and specificity of >99%
*Sensitivity and specificity for each variant type were determined using a collection of contrived positive samples and cfNA isolated from normal healthy donors.
Does the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay use AmpliSeq technology?
No, this assay uses Tag Sequencing technology. Cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are found at extremely low concentrations in the plasma fraction of whole blood. Because of this low prevalence, Taq Sequencing technology is utilized in this assay. The technology attaches unique molecular tags to the gene-specific primers. After amplification, the tagged molecules are grouped based on the tags. Groups containing the same mutant variant 80% of the time or greater will be called positive. Using the Tag technology, groups that contain random errors generated through the library construction/sequencing process are removed.