Gibco™

iPSC Lab Starter Kit with Reprogramming

Catalog number:  A32020

 Related applications:

Stem Cell Engineering & Reprogramming

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Description

The Gibco™ iPSC Lab Starter Kit with Reprogramming provides researchers initiating pluripotent stem cell research with the required reagents at a significant discount over purchasing them separately. This package solution is appropriate for labs that plan to perform reprogramming of donor-derived fibroblasts . For labs that are starting from cryopreserved pluripotent stem cells, please see the Gibco™ iPSC Lab Starter Kit, which does not include reprogramming reagents.

The iPSC Lab Starter Kit with Reprogramming includes the following products:

Reprogramming:
• CytoTune-iPS 2.0 Sendai Reprogramming Kit, Cat. No. A16517, 1 kit
• High glucose DMEM with GlutaMAX™-I, Cat. No. 10569-044, 40 x 500 mL
• Gibco™ Mouse Embryonic Fibroblasts (Irradiated), Cat. No. A24903, 11 vials
• MEM Non-Essential Amino Acids Solution (100X), Cat. No. 11140-050, 100 mL
• Fetal Bovine Serum, ES Cell-Qualified, Cat. No. 16141-061, 2 x 100 mL
• Attachment Factor, Cat. No. S-006-100, 2 x 100 mL
• 2-Mercaptoethanol (55 mM), Cat. No. 21985-023, 50 mL
• DMEM/F-12 with GlutaMAX™, Cat. No. 10565-018, 5 x 500 mL
• KnockOut™ Serum Replacement, Cat. No. 10828-010, 3 x 100 mL
• FGF-basic (AA 1-155) Recombinant Human Protein, Cat. No. PHG0264, 4 vials
• Collagenase Type IV, Cat. No. 17104-019, 1 vial
• Tra-1-60 AlexaFluor™ 488 Conjugate for Live Staining, Cat. No. A25618, 1 kit

PSC feeder-free transition and expansion:
• Essential 8™ Adaptation Kit, Cat. No. A25953, 1 kit
• Essential 8™ Medium, Cat. No. A1517001, 4 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 3 x 1 mL
• Versene Solution, Cat. No. 15040066, 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 20 x 500 mL

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells (Human), Stem Cells (iPS - Induced Pluripotent Stem)
Product Size: 1 kit
Shipping Condition: Dry Ice

Documents

Manuals & protocols