Invitrogen™

Countess™ II FL Automated Cell Counter Starter Package

Catalog number:  A32136

 Related applications:

Cell Counting, Viability, & Cryopreservation

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Description

The Countess II FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, and measure cell viability. This Countess II FL Starter Package contains the Countess II FL Automated Cell Counter, two of the most popular EVOS light cubes (GFP and RFP), disposable and reusable slides to maximize convenience and minimize cost, as well as trypan blue stain.

• Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
• Flexible—accepts up to two EVOS light cubes that are user-selectable from over twenty fluorescence channels
• Fast—counts live and dead cells and measures average cell size in as little as 10 seconds. Counting speed is virtually identical when using fluorescence.
• Convenient—requires no cleaning or routine maintenance. A capacitive touchscreen and simple user interface provides quick startup and requires minimal training.

Get accurate cell counts
The Countess II FL Automated Cell Counter uses trypan blue staining combined with an auto-focus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 × 104 to 1 × 107 cells/mL (the optimal range is 1 × 105 to 4 × 106 cells/mL), which is broader than that of a hemocytometer. The optimal cell size is 10–60 µm.

Simple operation with fluorescent application versatility
Operated using an intuitive user interface, automated cell counting with the Countess II FL instrument gives you the data you need to prepare your sample for the next passage or experiment, and allows you to multiplex with a variety of fluorescent dyes and proteins excited via EVOS light cubes.

Transfer data and images to your PC
Using the USB port on the Countess II FL Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. (For your convenience, a USB drive is supplied with the Countess II FL instrument.)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): EVOS™ FL Imaging System
Product Line: Countess™
Product Size: 1 package
Shipping Condition: Ambient Temperature
Type: Other Accessories

Contents & storage

Package includes:
• One Countess II FL Automated Cell Counter (AMQAF1000)
• Power cord with adaptors
• Quick reference guide
• USB drive
• Disposable (AMEP4745) & reusable (AMEP4746) slide holders
• Light cube removal tool (AMEP4747)
• 500 disposable slides (C10312)
• Reusable slide (A25750)
• EVOS GFP Light Cube (AMEP4651)
• EVOS RFP Light Cube (AMEP4652)

Documents

Manuals & protocols