ReproSeq™ PGS 318 View Kit, with chips - FAQs

Ion ReproSeq PGS 314 View Kit, without chips (16 samples): Cat. No. A32251
Ion ReproSeq PGS 316 View Kit, without chips (120 samples): Cat. No. A32252
Ion ReproSeq PGS 318 View Kit, without chips (192 samples): Cat. No. A32253
Ion ReproSeq PGS 314 View Kit, with chips (Up to 2 samples per chip, 8 chips included): Cat. No. A32243
Ion ReproSeq PGS 316 View Kit, with chips (Up to 15 samples per chip, 8 chips included): Cat. No. A32249
Ion ReproSeq PGS 318 View Kit, with chips (Up to 24 samples per chip, 8 chips included): Cat. No. A32250

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Here are the kit components:

Wash 2 Bottle w/label (2 L): 1 bottle
Wash 2 Bottle Conditioning Solution: 125 mL

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Here are the kit components:

Ion PGM Sequencing Supplies (Part No. A25587)
- Wash 1 Bottle w/label (250 mL): 1 bottle
- Wash 3 Bottle w/label (250 mL): 1 bottle
- Ion PGM Reagent Bottle Sipper Tubes (blue): 16 tubes
- Ion PGM Wash Bottle Sipper Tubes (gray): 8 tubes for 250-mL bottles; 4 tubes for 2-L bottles
- Reagent Bottles w/labels (50 mL): 25 bottles

Ion PGM Hi-Q View Sequencing Reagents (Part No. A30043)
- Ion PGM Hi-Q View Sequencing Polymerase (yellow cap): 36 µL
- Ion PGM Hi-Q View Sequencing Primer (white cap): 144 µL
- Ion PGM Hi-Q View Control Ion PGM Ion Sphere Particles (clear cap): 60 µL

Ion PGM Hi-Q View Sequencing Solutions (Part No. A30275)
- Ion PGM Hi-Q View Sequencing W2 Solution: 4 x 125 mL
- Ion Cleaning Tablet: 4 tablets
- Annealing Buffer: 12 mL
- Ion PGM Hi-Q View Sequencing W3 Solution: 2 x 100 mL

Ion PGM Hi-Q Sequencing dNTPs (Part No. A25590)
- Ion PGM Hi-Q Sequencing dGTP (black cap): 80 µL
- Ion PGM Hi-Q Sequencing dCTP (blue cap): 80 µL
- Ion PGM Hi-Q Sequencing dATP (green cap): 80 µL
- Ion PGM Hi-Q Sequencing dTTP (red cap): 80 µL

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Here are the kit components:

Ion PGM Template IA Supplies 500 (Part No. A24618)
- Ion OneTouch ES Supplies: 1 bag

Ion PGM Template IA Reagents 500 (Part No. A24619)
- Ion PGM Template IA Pellets 500: 4 foil pouches [1]
- Ion PGM Template IA ISP Dilution Buffer (yellow cap): 1 mL
- Ion PGM Template IA Start Solution (purple cap): 2 x 1200 µL

Ion PGM Template IA Reactions 500 (Part No. A24620)
- Ion PGM Template IA Ion Sphere Particles (orange cap): 86 µL
- Ion PGM Template IA Primer Mix S (black cap): 48 µL
- Ion PGM Template IA Primer Mix L (blue cap): 48 µL [2]
- Ion PGM Template IA Rehydration Buffer (white cap): 2 x 1.5 mL

Ion PGM Template IA Solutions (Part No. A24621)
- Ion PGM Template IA Stop Solution: 2.8 mL
- Ion PGM Template IA Recovery Solution: 7 mL
- Ion PGM Template IA Wash Solution: 4 mL
- MyOne Beads Wash Solution (green cap): 1.04 mL
- Neutralization Solution (red cap): 40 µL
- Tween Solution: 3.5 mL

Note: [1] One Ion PGM Template IA Pellet 500 per tube per pouch.
[2] Primer Mix L is intended for amplification of long templates and is not used with the Ion ReproSeq PGS View Kit

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Here are the kit components:

- Ion SingleSeq Kit (1, 5 or 8 boxes per Ion ReproSeq PGS View Kit, depending on the kit)
- Ion PGM Template IA 500 Kit (4 boxes/set, 1 set per Ion ReproSeq PGS View Kit)
- Ion PGM Hi-Q View Sequencing Kit (4 boxes/set, 1 set per Ion ReproSeq PGS View Kit)
- Ion PGM Wash 2 Bottle Kit (1 box per Ion ReproSeq PGS View Kit)
- Ion 314, Ion 316, or Ion 318 Chip Kit v2 BC (8 pack; Cat. Nos. A32243, A32249, A32250 only)

Note: The Ion SingleSeq Kit provides 24 unique Barcode Adapters and reagents to prepare 24 barcoded Ion SingleSeq libraries. The Ion PGM Template IA 500 Kit provides reagents and supplies to perform 4 isothermal amplification reactions. The Ion PGM Hi-Q View Sequencing Kit and Ion PGM Wash 2 Bottle Kit provide reagents and materials to perform 4 initializations and up to 12 sequencing runs on the Ion PGM Sequencer. Store the kit components as directed. See the Ion PGM Hi‑Q View Sequencing Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0014583_IonPGM_HiQ_View_Sequenc_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Please take a look at this page (http://www.thermofisher.com/us/en/home/life-science/sequencing/dna-sequencing/preimplantation-genetic-screening.html). Also, please see this application note (http://tools.thermofisher.com/content/sfs/brochures/Aneuploidy-App-Note.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Yes, the Ion ReproSeq PGS View Kits are a single-tube workflow, which minimizes sample loss and contamination.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion ReproSeq PGS View Kit on the Ion PGM System provides a simple, cost-effective, and scalable aneuploidy detection solution in a workflow as little as 8 hours long. After isolation of a single cell or multiple cells from a preimplantation embryo, DNA extraction and library construction with barcoding are performed using the Ion SingleSeq Kit in 3.5 hours. The template is then prepared using the Ion PGM Template IA 500 Kit in just 2 hours and loaded onto a chip. The sequencing run and analysis are completed in just 2.5 hours for the Ion 314 Chip or 4.5 hours for the Ion 318 Chip. Primary data analysis is performed using Torrent Suite Software, and aneuploidy status is determined using Ion Reporter Software.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.