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Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647 - FAQs

View additional product information for Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647 - FAQs (A32733, A32733TR)

4 product FAQs found

What is the degree of labelling (DOL), approximate number or range of fluorophores per IgG, of your antibody Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Cat. No. A32733, A32733TR)?

We do not report the actual measured dye ratios for Alexa Fluor Plus secondary antibodies on the Certificates of Analysis. Instead, we have a DOL range specification that is required for the conjugate to pass quality control. Alexa Fluor Plus 647 Goat anti-rabbit antibody lots are required to have a DOL within 5-8 dyes per antibody.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What mounting media should I use with samples labeled with Alexa Fluor Plus secondary antibodies?

All Alexa Fluor Plus secondary antibodies are compatible with the SlowFade Diamond and ProLong Diamond Antifade Mountants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I use Alexa Fluor Plus secondary antibodies relative to the original Alexa Fluor secondary antibodies?

Because they are brighter, you would most likely be able to use a lower final concentration of the Alexa Fluor Plus secondary antibodies relative to the original Alexa Fluor secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are the Alexa Fluor Plus secondary antibodies different from the original Alexa Fluor secondary antibodies?

Compared to existing Alexa Fluor secondary antibodies, the new Alexa Fluor Plus secondary antibodies use a new proprietary dye chemistry coupled to highly cross-adsorbed antibodies, offering higher sensitivity and signal-to-noise ratios.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.