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Alexa Fluor™ 546 Carboxylic Acid, tris(triethylammonium) salt

Catalog number:  A33079

Invitrogen™  Related applications: Protein Labeling & Crosslinking

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Alexa Fluor® 546 Carboxylic Acid is a non-reactive form of Alexa Fluor® 546 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 546 dye include:

• Laser line: 546 nm
• Common filter set: TRITC (tetramethylrhodamine)
• Excitation maxima: 556 nm
• Emission maxima: 573 nm

More About Alexa Fluor® 546 Dye
Alexa Fluor® 546 dye is a bright, yellow-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. Alexa Fuor® 546 serves as an alternative for tetramethylrhodamine or Cy®3 dye, with higher initial intensity than Alexa Fluor® 555 dye, but lower photostability in buffer or antifade. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: Alexa Fluor® 546
Product Line: Alexa Fluor™
Product Size: 5 mg

Contents & storage

Store in freezer (-5 to -30°C). Protect from light.


Manuals & protocols

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