Molecular Probes® Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 0.5–1 mg of antibody conjugate. Purification is accomplished using an easy-to-assemble gravity-fed size-exclusion column containing BioGel® P-30 resin. Each kit contains sufficient material for 3 separate purifications, and can also be used to purify high-molecular weight proteins (> 40 kDa) away from unconjugated labels.
Additional Features of this Antibody Conjugate Purification Kit:
• Purified conjugate ready-to-use in ∼45 minutes with ∼15 minutes of hands-on time • Designed for low-molecular weight labels (<3000 Da, e.g. Alexa Fluor® dyes); cannot be used for removing unconjugated high-molecular weight labels (>10 kDa) such as enzymes (HRP) or proteins (R-phycoerythrin)
More about Protein and Antibody Labeling We offer a wide selection of Molecular Probes® antibody and protein labeling kits to fit your starting material and your experimental set-up. To learn more about our labeling kits, see Kits for Labeling Proteins and Nucleic Acids in The Molecular Probes® Handbook or visit our antibody labeling page.
For Research Use Only. Not for use in diagnostic procedures.
Contents & Storage
Store in refrigerator (2–8°C).
Antibody Conjugate Purification Column for 0.5-1 mg Assembly