Molecular Probes® Antibody Conjugate Purification kits efficiently purify antibody conjugates from unconjugated dye or biotin. This particular kit is optimized for the purification of 20–50 µg of antibody conjugate. Purification is accomplished using an easy-to-assemble size-exclusion spin column containing BioGel® P-6 fine resin. Each kit contains sufficient material for 3 separate purifications, and can also be used to purify proteins with a molecular weight greater than 12 kDa away from unconjugated labels.
Additional Features of this Antibody Conjugate Purification Kit:
• Purified conjugate ready-to-use in ∼10 minutes with ∼5 minutes of hands-on time
• Designed for low-molecular weight labels (<3000 Da, e.g. Alexa Fluor® dyes); cannot be used for removing unconjugated high-molecular weight labels (>8 kDa) such as enzymes (HRP) or proteins (R-phycoerythrin)