The Feed Kit A+, B+, C+ provides three supplements and a supplementation strategy designed to optimize cell growth and/or productivity in CHO cells. The three supplements, EfficientFeed™ A+, EfficientFeed™ B+, and EfficientFeed™ C+ are based on the chemically defined CHO CD EfficientFeed™ A, CHO CD EfficientFeed™ B, and CD EfficientFeed™ C nutrient supplements. Gibco™ EfficientFeed+ AGT™ supplements are dry-format, single-part supplements for fed-batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to the original EfficientFeed AGT nutrient supplements, EfficientFeed+ supplements have components that are uniquely combined to allow simpler single-part pH-neutral reconstitutions, as well as the ability to super-concentrate the supplements for reduction of product dilution or increased titers with additional feeding. By using the three feed supplements independently or in combination, you can optimize fed-batch protocols for both cell growth and protein yield.
Criteria for supplementation
Specific CHO cell lines and clones react differently to alternative supplementation protocols. Metabolic needs vary and it is essential for optimal growth and/or productivity to determine optimal nutrient supplementation protocols for each cell line or clone. You may find that two or more different feeds are needed. General criteria to consider when designing supplementation protocol experiments are provided in the user manual (downloadable below).
Selecting the feed or combination that may fit best for your process
A chart is provided below to help in the selection of Gibco media and feeds to pair for testing based on cell type. Should you wish to gain additional insight into different methods as well as the field support available to you, consult the user guide or request support through your local Account Manager.
Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may lessen the chance of contamination by reducing the number of connections to the bioreactor to one or two . Customization of other feeding formulations is possible should that option suit your needs better after trying this kit. You can contact your local Field Application Scientist through your Account Manager to learn more about our customization capabilities.