Thermo Scientific™

RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit

 Related applications:
Food & Beverage
Industrial Microbiology

Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with Thermo Scientific™ RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit. Be confident in your test accuracy and speed up product release with results in as little as 16 to 22 hours and validated workflows for poultry and pork meats, and production environment samples. The simple-to-use kit is compatible with the Applied Biosystems™ 7500 Fast Food Safety or Applied Biosystems™ Quant Studio 5 Food Safety Real-Time PCR systems.

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Description

Increase your workflow efficiency while meeting regulatory requirements to detect the most common Salmonella serovars which cause disease in humans with the RapidFinder Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit. The real-time PCR kit enables rapid, simultaneous detection and presumptive identification of Salmonella species, Salmonella Typhimurium, and Salmonella Enteritidis in a single well, with AOAC-RI validated workflows on a range of matrices (chicken thighs, chicken wings, chicken nuggets, pork sausage and stainless steel sponges).

  • Faster decisions: earlier product release (negative results) and quicker interventions (positive results) due to simultaneous detection (three tests in one) and a reduced time-to-result compared with culture methods.
  • Cost reductions: up to 50% consumable cost savings compared with running individual PCR tests for Salmonella species, Salmonella Typhimurium, and Salmonella Enteritidis.
  • Increased efficiency: save valuable technician time with single, non-proprietary enrichment, ready-to-use reagents, simple sample preparation and simultaneous identification.
  • Confident results: Applied Biosystems™ TaqMan™ Real-Time PCR probes provide a high level of specificity and sensitivity for industry relevant serovars. Internal amplification controls give added confidence.
  • Convenience: part of a range of end-to-end (enrichment to confirmation) testing solutions including all microbiology reagents and instruments, along with dedicated technical support worldwide. 

Kit includes:

  • Pre-dispensed and ready-to-use reagents for cell lysis and release of DNA from bacteria present in enriched food and environmental samples.
  • Pre-dispensed lyophilized PCR pellets containing target-specific primers, probes, Internal Positive Control (IPC) and reagents for multiplex real-time PCR using the prepared lysate.

Compatible systems and software:

  • Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR Instrument with Applied Biosystems™ RapidFinder™ Express Software v2.0 or higher.
    • A30304 (desktop)
    • A30299 (laptop)
  • Applied Biosystems™ QuantStudio 5 (0.1 ml) Food Safety Real-Time PCR Instrument with Thermo Scientific™ RapidFinder™ Analysis Software v1.0 or higher.
    • A36320 (desktop)
    • A36328 (laptop)
  • Applied Biosystems™ SimpliAmp™ Thermal Cycler – A24811


Documents

Manuals & protocols