The Applied Biosystems VetMAX PEDV/TGEV/SDCoV Kit is a single-well multiplex real-time RT-PCR kit for the detection and differentiation of the porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and swine delta corona virus (SDCoV) in environmental samples.
• 4 separate tests are run per sample in 1 well—no need to run several PCRs per virus • All components needed to run a PCR are included in the kit for ease of ordering • The amplification can be run in standard and fast mode, depending on your needs and laboratory workflow • Instructions included
The kit includes: • TaqMan Fast Virus 1-Step Master Mix • VetMAX PEDV/TGEV/SDCoV Primer Probe Mix—for optimized multiplex real-time RT-PCR amplification of PEDV/TGEV/SDCoV RNA targets • VetMAX PEDV/TGEV/SDCoV/Xeno Control RNA Mix—serves as a positive control for the real-time RT-PCR components and is also used to set the cycle threshold (CT) for evaluating test results • VetMAX Xeno Positive Control RNA—serves as an internal positive control for the RNA purification process and a monitor for the presence of PCR inhibitors
PEDV, TGEV, and SDCoV cause acute and contagious enteric diseases in swine that are characterized by diarrhea, fever, and vomiting. These diseases can cause significant morbidity and mortality, particularly in young piglets, resulting in economic losses in the swine industry.
PED, TGE, and SDCo have clinical signs that are similar and so need confirmation by laboratory diagnostics to differentiate them. In most cases PED spreads more slowly on the farm than TGE. The viruses can be easily spread through exposure to contaminated vomit and diarrhea in the environment, so helping to control biosecurity by testing environmental samples from barns and transport vehicles will increase the prevention of disease outbreaks on farms.
Ease of use All components for running the PCR are included in the kit. The sample preparation workflow has been tested with Applied Biosystems MagMAX sample extraction chemistry and Thermo Fisher KingFisher instruments. The kit has been validated for several sample types, eliminating the need for separate kits for each type. And this real-time PCR kit can be used with different thermocyclers, with the same results and reliability.
Contact us for more information on how to collect environmental samples on the farm or in transport vehicles.
Environmental samples are: • Feed samples • Fecal samples collected from floors or walls in the pen or barn • Swiffer pads • Any sample not directly collected from individual pigs
For Testing of Food and Environmental Samples Only.
Swiffer pads and boots, Food & Environmental Samples