Thermo Scientific™

Mass Spectrometry Cleavable Crosslinkers

Catalog number: A33545
Thermo Scientific™

Mass Spectrometry Cleavable Crosslinkers

Catalog number: A33545
Chemical crosslinking in combination with mass spectrometry is a powerful method to improve protein characterization and identification of protein-protein interactions. This method has been applied to recombinant and native protein complexes and, more recently, to whole cell lysates or intact unicellular organisms in efforts to identify protein-protein interactions on a global scale. The more recent enhancement of cleavability in DSSO and DSBU has improved MS compatibility and sensitivity of detection of crosslinked peptides and proteins.
 
Catalog Number
A33545
Unit Size
10 x 1 mg
Description
DSSO
Price (USD)
Price: 357.00
Your Price:
Availability
-
Full specifications
Quantity
Chemical ReactivityAmine-Amine
CleavableBy CID using MS
DescriptionDSSO
Molecular Weight388.35
PEGylatedNo
Spacer Arm Length10.3 Å
Cell-PermeableYes
Labeling MethodChemical Labeling
Crosslinker TypeHomobifuntional
Reactive MoietyNHS Ester
SpacerMedium (10 to 30 Å)
FormPowder
Quantity10 x 1 mg
SolubilityDMF, DMSO
FormatStandard, Single use
Water-SolubleNo
Product TypeCrosslinker
Unit Size10 x 1 mg
Catalog Number
Unit Size
10 x 1 mg
Description
DBSU
Price (USD)
Price: 365.00
Your Price:
Availability
-
Full specifications
Quantity
Chemical ReactivityAmine-Amine
CleavableBy CID using MS
DescriptionDBSU
Molecular Weight426.38
PEGylatedNo
Spacer Arm Length12.5 Å
Cell-PermeableYes
Shipping ConditionWet Ice
Labeling MethodChemical Labeling
Crosslinker TypeHomobifuntional
Reactive MoietyNHS Ester
SpacerMedium (10 to 30 Å)
FormPowder
Quantity10 x 1 mg
SolubilityDMF, DMSO
FormatStandard, Single use
Water-SolubleNo
Product TypeCrosslinker
Unit Size10 x 1 mg
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
A33545Full specifications
10 x 1 mgDSSO
Price: 357.00
Your Price:
-
Chemical ReactivityAmine-Amine
CleavableBy CID using MS
DescriptionDSSO
Molecular Weight388.35
PEGylatedNo
Spacer Arm Length10.3 Å
Cell-PermeableYes
Labeling MethodChemical Labeling
Crosslinker TypeHomobifuntional
Reactive MoietyNHS Ester
SpacerMedium (10 to 30 Å)
FormPowder
Quantity10 x 1 mg
SolubilityDMF, DMSO
FormatStandard, Single use
Water-SolubleNo
Product TypeCrosslinker
Unit Size10 x 1 mg
A35459Full specifications
10 x 1 mgDBSU
Price: 365.00
Your Price:
-
Chemical ReactivityAmine-Amine
CleavableBy CID using MS
DescriptionDBSU
Molecular Weight426.38
PEGylatedNo
Spacer Arm Length12.5 Å
Cell-PermeableYes
Shipping ConditionWet Ice
Labeling MethodChemical Labeling
Crosslinker TypeHomobifuntional
Reactive MoietyNHS Ester
SpacerMedium (10 to 30 Å)
FormPowder
Quantity10 x 1 mg
SolubilityDMF, DMSO
FormatStandard, Single use
Water-SolubleNo
Product TypeCrosslinker
Unit Size10 x 1 mg
Showing 2 of 2
• Highly reactive NHS ester (at both ends) with primary amines
• MS-cleavable by collision-induced dissociation (CID)
• High-purity crystalline reagents for protein structure and interaction characterization
• Membrane-semi-permeable, allowing intracellular crosslinking
• Water-insoluble (dissolve first in DMF or DMSO)

DSSO (disuccinimidyl sulfoxide) and DSBU (disuccinimidyl dibutyric urea) (or BuUrBU) are both MS-cleavable and membrane-semi-permeable crosslinkers that contain an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm (DSSO) or 11-atom spacer arm (DSBU). Both crosslinkers have similar reactivity to DSS but contain a cleavable functionality in the linker region that enables cleavage in the gas phase using collision-induced dissociation (CID) during tandem MS (MS/MS) fragmentation. The MS cleavage of DSSO or DSBU generates diagnostic ion doublets during MS2, which distinguishes identification of cross-linked peptides from dead-end modifications. The crosslinked peptide fragments are subsequently identified using MeroX or XlinkX software. For DSSO, cleavage of cross-linked peptides during MS/MS enables MS3 acquisition methods, which additionally facilitates peptide sequencing using traditional database search engines.

Note: For high resolution analysis of the crosslinked peptides, the recommended LC column for the Nanospray Flex source is the Acclaim PepMap 100 C18 LC Column (Cat. No. 164940 or 164568). For the EASY-Spray source, the recommended LC column is the EASY-Spray C18 LC Column (Cat. No. ES900 or ES904).

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Upon receipt store at 4°C protected from moisture.