Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (2 sequencing runs per initialization)
Ion Torrent™

Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (2 sequencing runs per initialization)

The Ion 510 & Ion 520 & Ion 530 Kit-Chef (2 sequencing runs per initialization) enables robust and automated templateMás información
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Número de catálogoCantidad
A344618 reactions
Número de catálogo A34461
Precio (USD)
3.696,46
Each
Cantidad:
8 reactions
Precio (USD)
3.696,46
Each
The Ion 510 & Ion 520 & Ion 530 Kit-Chef (2 sequencing runs per initialization) enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system. The Ion 510 & Ion 520 & Ion 530 Kit contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. This kit accommodates 2 sequencing runs per initialization. For 1 sequencing run per initialization (required for 400 base-read applications), see Cat. No. A34019.

The Ion 510 & Ion 520 & Ion 530 Kit-Chef workflow:
• Provides template preparation and chip loading reagents for up to 200 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for the Ion 510, Ion 520, and Ion 530 chips

The Ion S5 and Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 510 & Ion 520 & Ion 530 Kit with the Ion 510 Chip Kit (up to 3 million reads per chip), the Ion 520 Chip Kit (up to 5 million reads per chip), or Ion 530 Chip Kit (up to 20 million reads), allowing for flexibility of total throughput. For even greater flexibility, combine different chip types in a single Ion Chef run when using Torrent Suite Software 5.6 and above. For de novo research applications, add the Ion S5 Calibration Standard to prepared libraries for improved base-calling accuracy.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Línea de productosIon Chef, Ion Torrent
Cantidad8 reactions
Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo húmedo
Unit SizeEach
Contenido y almacenamiento
• Ion S5 Chef supplies, store at room temperature
• Ion S5 Chef solutions, store at room temperature
• Ion 510 and Ion 520 and Ion 530 Chef reagents, store at -5 to -30°C
• Ion S5 Sequencing solutions, store at room temperature
• Ion S5 Sequencing reagents, store at -5 to -30°C

Preguntas frecuentes

How long do Ion S5 sequencing reagents from the ION 510/520/530 Kit-Chef (2R/I) (1 Kit) need to thaw?

The Ion S5 Sequencing Reagents cartridge (Part No. A27768) should equilibrate to room temperature for 45 minutes before use.

How many samples can we test with the ION 510/520/530 Kit-Chef (2R/I) (1 Kit)?

The kit contains reagents for templating, chip loading, and sequencing up to 8 libraries. The total number of samples you can test depends on how many samples you multiplex per library.

How many 520 chips can be sequenced with the whole ION 510/520/530 Kit-Chef (2R/I) (1 Kit) (8 reactions)?

The kit supports sequencing of up to 8 Ion 520 Chips.

How long does the reagent cartridge from the ION 510/520/530 Kit-Chef (2R/I) (1 Kit) need to equilibrate to room temperature?

The Ion 510/520/530 Chef Reagents cartridge (Part No. A34018) should equilibrate to room temperature for 45 minutes before use.

Why did my Ion Chef Instrument stop during a run?

- The instrument may have encountered an error. We recommend recording the error shown on the instrument display, then tapping OK. For further assistance, contact Technical Support at techsupport@thermofisher.com.
- A consumable may have been improperly loaded. We recommend ensuring that all consumables are correctly loaded. For instructions, see the user guide for your templating kit. For further assistance, contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.