The Applied Biosystems TaqMan Advanced miRNA Human Endogenous Controls Card provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains one card than can be used to evaluate 30 potential endogenous control miRNAs in triplicate for up to four samples. Two exogenous (non-human) controls are included for normalization of sample input.

Features of the TaqMan Advanced miRNA Human Endogenous Controls Card include:
• Reproducibility—pre-spotted assays help reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL purified plasma/serum or 1–10 ng total RNA per sample
• Updated content—assay content updated for mirBase v.22
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:

Number of cards: 1
Number of unique miRNAs (not including exogenous controls): 30
Exogenous controls: cel-miR-39-3p, ath-miR159a
Compatible with the following Applied Biosystems qPCR instruments: Viia7, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA cards require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses a miRNA-specific reverse transcription chemistry.