Use with Annexin Binding Buffer for flow cytometry
Invitrogen™

Annexin V Conjugates for Apoptosis Detection

Catalog number: A35122
Invitrogen™

Annexin V Conjugates for Apoptosis Detection

Catalog number: A35122
Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry.
 
Catalog Number
A35122
Unit Size
500 µL
Conjugate
Pacific Blue™
Excitation/Emission
410/455
Flow Cytometer Laser Lines
405
Price (USD)
375.00
Your Price:
Availability
***
Full specifications
Quantity
ColorBlue
DescriptionAnnexin V, Pacific Blue conjugate, for flow cytometry
Excitation/Emission410/455
Flow Cytometer Laser Lines405
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugatePacific Blue™
Kit ContentsContains 1 vial of annexin V, Pacific Blue conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 488
Excitation/Emission
495/519
Flow Cytometer Laser Lines
488
Price (USD)
342.00
Your Price:
Availability
***
Full specifications
Quantity
ColorGreen
DescriptionAnnexin V, Alexa Fluor 488 conjugate (replacement for Cat. No. PHN1010 and PHN1008)
Excitation/Emission495/519
Flow Cytometer Laser Lines488
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 488
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 488 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
FITC
Excitation/Emission
494/518
Flow Cytometer Laser Lines
488
Price (USD)
342.00
Your Price:
Availability
***
Full specifications
Quantity
ColorGreen
DescriptionAnnexin V, FITC conjugate (replaces AnnexinV01, AnnexinV013, PHN1010, PHN1008)
Excitation/Emission494/518
Flow Cytometer Laser Lines488
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateFITC
Kit ContentsContains 1 vial of annexin V, FITC conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 568
Excitation/Emission
578/603
Flow Cytometer Laser Lines
532, 561
Price (USD)
377.00
Your Price:
Availability
***
Full specifications
Quantity
ColorOrange-Red
DescriptionAnnexin V, Alexa Fluor 568 conjugate
Excitation/Emission578/603
Flow Cytometer Laser Lines532, 561
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 568
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 568 conjugate.

Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 594
Excitation/Emission
590/617
Flow Cytometer Laser Lines
532
Price (USD)
377.00
Your Price:
Availability
***
Full specifications
Quantity
ColorRed
DescriptionAnnexin V, Alexa Fluor 594 conjugate
Excitation/Emission590/617
Flow Cytometer Laser Lines532
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 594
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 594 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Biotin-X
Excitation/Emission
Flow Cytometer Laser Lines
Price (USD)
377.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionAnnexin V, biotin-X conjugate
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateBiotin-X
Kit ContentsContains 1 vial of annexin V, biotin-x conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 350
Excitation/Emission
346/442
Flow Cytometer Laser Lines
UV
Price (USD)
371.00
Your Price:
Availability
***
Full specifications
Quantity
ColorBlue
DescriptionAnnexin V, Alexa Fluor 350 conjugate
Excitation/Emission346/442
Flow Cytometer Laser LinesUV
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 350
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 350 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 647
Excitation/Emission
650/665
Flow Cytometer Laser Lines
633-637
Price (USD)
377.00
Your Price:
Availability
***
Full specifications
Quantity
ColorFar-Red
DescriptionAnnexin V, Alexa Fluor 647 conjugate
Excitation/Emission650/665
Flow Cytometer Laser Lines633-637
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 647
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 647 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 555
Excitation/Emission
555/565
Flow Cytometer Laser Lines
532, 561
Price (USD)
377.00
Your Price:
Availability
***
Full specifications
Quantity
ColorOrange
DescriptionAnnexin V, Alexa Fluor 555 conjugate
Excitation/Emission555/565
Flow Cytometer Laser Lines532, 561
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 555
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 555 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Catalog Number
Unit Size
500 µL
Conjugate
Alexa Fluor™ 680
Excitation/Emission
679/702
Flow Cytometer Laser Lines
633-637
Price (USD)
377.00
Your Price:
Availability
***
Full specifications
Quantity
ColorNear IR
DescriptionAnnexin V, Alexa Fluor 680 conjugate
Excitation/Emission679/702
Flow Cytometer Laser Lines633-637
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 680
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 680 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Showing 10 of 12Show more
Catalog NumberSpecificationsUnit SizeConjugateExcitation/EmissionFlow Cytometer Laser LinesPrice (USD)AvailabilityQuantity
A35122Full specifications
500 µLPacific Blue™410/455405
375.00
Your Price:
***
ColorBlue
DescriptionAnnexin V, Pacific Blue conjugate, for flow cytometry
Excitation/Emission410/455
Flow Cytometer Laser Lines405
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugatePacific Blue™
Kit ContentsContains 1 vial of annexin V, Pacific Blue conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A13201Full specifications
500 µLAlexa Fluor™ 488495/519488
342.00
Your Price:
***
ColorGreen
DescriptionAnnexin V, Alexa Fluor 488 conjugate (replacement for Cat. No. PHN1010 and PHN1008)
Excitation/Emission495/519
Flow Cytometer Laser Lines488
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 488
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 488 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A13199Full specifications
500 µLFITC494/518488
342.00
Your Price:
***
ColorGreen
DescriptionAnnexin V, FITC conjugate (replaces AnnexinV01, AnnexinV013, PHN1010, PHN1008)
Excitation/Emission494/518
Flow Cytometer Laser Lines488
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateFITC
Kit ContentsContains 1 vial of annexin V, FITC conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A13202Full specifications
500 µLAlexa Fluor™ 568578/603532, 561
377.00
Your Price:
***
ColorOrange-Red
DescriptionAnnexin V, Alexa Fluor 568 conjugate
Excitation/Emission578/603
Flow Cytometer Laser Lines532, 561
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 568
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 568 conjugate.

Product TypeAnnexin V conjugate
Unit Size500 µL
A13203Full specifications
500 µLAlexa Fluor™ 594590/617532
377.00
Your Price:
***
ColorRed
DescriptionAnnexin V, Alexa Fluor 594 conjugate
Excitation/Emission590/617
Flow Cytometer Laser Lines532
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 594
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 594 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A13204Full specifications
500 µLBiotin-X
377.00
Your Price:
***
DescriptionAnnexin V, biotin-X conjugate
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateBiotin-X
Kit ContentsContains 1 vial of annexin V, biotin-x conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A23202Full specifications
500 µLAlexa Fluor™ 350346/442UV
371.00
Your Price:
***
ColorBlue
DescriptionAnnexin V, Alexa Fluor 350 conjugate
Excitation/Emission346/442
Flow Cytometer Laser LinesUV
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 350
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 350 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A23204Full specifications
500 µLAlexa Fluor™ 647650/665633-637
377.00
Your Price:
***
ColorFar-Red
DescriptionAnnexin V, Alexa Fluor 647 conjugate
Excitation/Emission650/665
Flow Cytometer Laser Lines633-637
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 647
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 647 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A35108Full specifications
500 µLAlexa Fluor™ 555555/565532, 561
377.00
Your Price:
***
ColorOrange
DescriptionAnnexin V, Alexa Fluor 555 conjugate
Excitation/Emission555/565
Flow Cytometer Laser Lines532, 561
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 555
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 555 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
A35109Full specifications
500 µLAlexa Fluor™ 680679/702633-637
377.00
Your Price:
***
ColorNear IR
DescriptionAnnexin V, Alexa Fluor 680 conjugate
Excitation/Emission679/702
Flow Cytometer Laser Lines633-637
For Use With (Equipment)Flow Cytometer
No. of Reactions100
Shipping ConditionWet Ice
ConjugateAlexa Fluor™ 680
Kit ContentsContains 1 vial of annexin V, Alexa Fluor 680 conjugate.
Product TypeAnnexin V conjugate
Unit Size500 µL
Showing 10 of 12Show more
Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.

The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.

The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits

Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in refrigerator (2°C to 8°C) and protect from light.

Figures

Frequently asked questions (FAQs)

Citations & References